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  A new MDCK suspension line cultivated in a fully defined medium in stirred-tank and wave bioreactor

Lohr, V., Genzel, Y., Behrendt, I., Scharfenberg, K., & Reichl, U. (2010). A new MDCK suspension line cultivated in a fully defined medium in stirred-tank and wave bioreactor. Vaccine, 28(38), 6256-6264. doi:10.1016/j.vaccine.2010.07.004.

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 Urheber:
Lohr, V.1, Autor           
Genzel, Y.1, Autor           
Behrendt, I.1, Autor           
Scharfenberg, K.2, Autor
Reichl, U.1, 3, Autor           
Affiliations:
1Bioprocess Engineering, Max Planck Institute for Dynamics of Complex Technical Systems, Max Planck Society, ou_1738140              
2University of Applied Sciences Emden/Leer, Emden, ou_persistent22              
3Otto-von-Guericke-Universität Magdeburg, ou_1738156              

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Schlagwörter: Influenza virus Vaccine production Suspension MDCK Chemically defined medium Wave bioreactor
 Zusammenfassung: An adherently growing MDCK cell line was adapted in a two-step process in a fully defined medium and in suspension. The resulting MDCK.SUS2 cells were subsequently evaluated for their potential as host cells for influenza vaccine production in two lab-scale bioreactors (wave and stirred-tank). Cell concentrations up to 2.3×106 cells/mL were obtained after 96 h, which is slightly higher than cell concentrations obtained with adherent MDCK cells cultivated on microcarriers (2 g/L). Infections with influenza A/PR/8/34 and B/Malaysia resulted in high virus titers (2.90 and 2.75 logHAunits/100uL, respectively). The monitoring of extracellular metabolites, including amino acids, revealed a change in some of the metabolite consumption or release profiles, which indicates changes in metabolism during the adaptation process. Overall, the MDCK.SUS2 cell line represents a new cell substrate for a robust influenza vaccine production in a fully defined process. © 2010 Elsevier Ltd. All rights reserved. [accessed September 21st, 2010]

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Sprache(n): eng - English
 Datum: 2010
 Publikationsstatus: Erschienen
 Seiten: -
 Ort, Verlag, Ausgabe: -
 Inhaltsverzeichnis: -
 Art der Begutachtung: Expertenbegutachtung
 Identifikatoren: eDoc: 494474
Anderer: 23/10
DOI: 10.1016/j.vaccine.2010.07.004
 Art des Abschluß: -

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Titel: Vaccine
Genre der Quelle: Zeitschrift
 Urheber:
Affiliations:
Ort, Verlag, Ausgabe: Guildford, Surrey, UK : Elsevier
Seiten: - Band / Heft: 28 (38) Artikelnummer: - Start- / Endseite: 6256 - 6264 Identifikator: ISSN: 0264-410X
CoNE: https://pure.mpg.de/cone/journals/resource/954925498091