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Abstract:
Mammalian cells play a major role in pharmaceutical industry and are essential for the
production of glycosylated proteins. For industrial application productivity is of main
interest.
In this study the productivity of the human cell line AGE1.HN.AAT[1] under different
experimental conditions is investigated. Cells were cultivated in shaker flasks for 70 h
using standard cultivation conditions at 37°C and an initial glucose concentration of
30 mM. In the late exponential growth phase cultivation conditions were changed.
As described in literature reduction of temperature resulted in higher product titers. In
the absence of glucose, the cell concentration remained constant for about 30 h at a
high viability. Interestingly, the cells still produced AAT. Experimental findings will be
discussed based on a comprehensive model using metabolic flux analysis.
