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Abstract:
Hybridization of nucleic acids on solid surfaces is a
key process involved in high-throughput technologies
such as microarrays and, in some cases, nextgeneration
sequencing (NGS). A physical understanding
of the hybridization process helps to determine
the accuracy of these technologies. The goal of a
widespread research program is to develop reliable
transformations between the raw signals reported
by the technologies and individual molecular concentrations
from an ensemble of nucleic acids. This
research has inputs from many areas, from bioinformatics
and biostatistics, to theoretical and experimental
biochemistry and biophysics, to computer
simulations. A group of leading researchers met
in Ploen Germany in 2011 to discuss present knowledge
and limitations of our physico-chemical
understanding of high-throughput nucleic acid
technologies. This meeting inspired us to write this
summary, which provides an overview of the
state-of-the-art approaches based on physicochemical
foundation to modeling of the nucleic
acids hybridization process on solid surfaces. In
addition, practical application of current knowledge
is emphasized.
