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  An asymmetric SMC-kleisin bridge in prokaryotic condensin

Bürmann, F., Shin, H.-C., Basquin, J., Soh, Y.-M., Giménez-Oya, V., Kim, Y.-G., et al. (2013). An asymmetric SMC-kleisin bridge in prokaryotic condensin. NATURE STRUCTURAL & MOLECULAR BIOLOGY, 20(3), 371-379. doi:10.1038/nsmb.2488.

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Bürmann, Frank1, Autor           
Shin, Ho-Chul2, Autor
Basquin, Jerome3, Autor           
Soh, Young-Min2, Autor
Giménez-Oya, Victor1, Autor           
Kim, Yeon-Gil2, Autor
Oh, Byung-Ha2, Autor
Gruber, Stephan1, Autor           
Affiliations:
1Gruber, Stephan / Chromosome Organization and Dynamics, Max Planck Institute of Biochemistry, Max Planck Society, ou_1565151              
2external, ou_persistent22              
3Conti, Elena / Structural Cell Biology, Max Planck Institute of Biochemistry, Max Planck Society, ou_1565144              

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Schlagwörter: PROMOTES CHROMOSOME SEGREGATION; SISTER-CHROMATID COHESION; ESCHERICHIA-COLI; BACILLUS-SUBTILIS; ATP HYDROLYSIS; DNA; PROTEINS; COMPLEX; ARCHITECTURE; BACTERIAL
 Zusammenfassung: Eukaryotic structural maintenance of chromosomes (SMC)-kleisin complexes form large, ring-shaped assemblies that promote accurate chromosome segregation. Their asymmetric structural core comprises SMC heterodimers that associate with both ends of a kleisin subunit. However, prokaryotic condensin Smc-ScpAB is composed of symmetric Smc homodimers associated with the kleisin ScpA in a postulated symmetrical manner. Here, we demonstrate that Smc molecules have two distinct binding sites for ScpA. The N terminus of ScpA binds the Smc coiled coil, whereas the C terminus binds the Smc ATPase domain. We show that in Bacillus sub fills cells, an Smc dimer is bridged by a single ScpAB to generate asymmetric tripartite rings analogous to eukaryotic SMC complexes. We define a molecular mechanism that ensures asymmetric assembly, and we conclude that the basic architecture of SMC-kleisin rings evolved before the emergence of eukaryotes.

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Sprache(n): eng - English
 Datum: 2013-03
 Publikationsstatus: Erschienen
 Seiten: 9
 Ort, Verlag, Ausgabe: -
 Inhaltsverzeichnis: -
 Art der Begutachtung: Expertenbegutachtung
 Identifikatoren: ISI: 000316041000020
DOI: 10.1038/nsmb.2488
 Art des Abschluß: -

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Titel: NATURE STRUCTURAL & MOLECULAR BIOLOGY
Genre der Quelle: Zeitschrift
 Urheber:
Affiliations:
Ort, Verlag, Ausgabe: 75 VARICK ST, 9TH FLR, NEW YORK, NY 10013-1917 USA : NATURE PUBLISHING GROUP
Seiten: - Band / Heft: 20 (3) Artikelnummer: - Start- / Endseite: 371 - 379 Identifikator: ISSN: 1545-9993