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  Deep transcriptome-sequencing and proteome analysis of the hydrothermal vent annelid Alvinella pompejana identifies the CvP-bias as a robust measure of eukaryotic thermostability

Holder, T., Basquin, C., Ebert, J., Randel, N., Jollivet, D., Conti, E., et al. (2013). Deep transcriptome-sequencing and proteome analysis of the hydrothermal vent annelid Alvinella pompejana identifies the CvP-bias as a robust measure of eukaryotic thermostability. BIOLOGY DIRECT, 8: 2. doi:10.1186/1745-6150-8-2.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-000E-ED5E-5 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-000E-ED5F-3
Genre: Journal Article

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1745-6150-8-2.pdf (Any fulltext), 2MB
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 Creators:
Holder, Thomas1, Author
Basquin, Claire2, Author              
Ebert, Judith2, Author              
Randel, Nadine1, Author
Jollivet, Didier1, Author
Conti, Elena2, Author              
Jekely, Gaspar1, Author
Bono, Fulvia1, Author              
Affiliations:
1external, escidoc:persistent22              
2Conti, Elena / Structural Cell Biology, Max Planck Institute of Biochemistry, Max Planck Society, escidoc:1565144              

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Free keywords: INTERSTITIAL COLLAGENS; MESOPHILIC PROTEINS; THERMAL-STABILITY; HIGH-THROUGHPUT; WORMS; EVOLUTION; HYPERTHERMOSTABILITY; THERMOPHILE; INSIGHTS; PROGRAM
 Abstract: Background: Alvinella pompejana is an annelid worm that inhabits deep-sea hydrothermal vent sites in the Pacific Ocean. Living at a depth of approximately 2500 meters, these worms experience extreme environmental conditions, including high temperature and pressure as well as high levels of sulfide and heavy metals. A. pompejana is one of the most thermotolerant metazoans, making this animal a subject of great interest for studies of eukaryotic thermoadaptation. Results: In order to complement existing EST resources we performed deep sequencing of the A. pompejana transcriptome. We identified several thousand novel protein-coding transcripts, nearly doubling the sequence data for this annelid. We then performed an extensive survey of previously established prokaryotic thermoadaptation measures to search for global signals of thermoadaptation in A. pompejana in comparison with mesophilic eukaryotes. In an orthologous set of 457 proteins, we found that the best indicator of thermoadaptation was the difference in frequency of charged versus polar residues (CvP-bias), which was highest in A. pompejana. CvP-bias robustly distinguished prokaryotic thermophiles from prokaryotic mesophiles, as well as the thermophilic fungus Chaetomium thermophilum from mesophilic eukaryotes. Experimental values for thermophilic proteins supported higher CvP-bias as a measure of thermal stability when compared to their mesophilic orthologs. Proteome-wide mean CvP-bias also correlated with the body temperatures of homeothermic birds and mammals. Conclusions: Our work extends the transcriptome resources for A. pompejana and identifies the CvP-bias as a robust and widely applicable measure of eukaryotic thermoadaptation.

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Language(s): eng - English
 Dates: 2013-01-16
 Publication Status: Published in print
 Pages: 16
 Publishing info: -
 Table of Contents: -
 Rev. Method: Peer
 Identifiers: ISI: 000315729000002
DOI: 10.1186/1745-6150-8-2
 Degree: -

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Title: BIOLOGY DIRECT
Source Genre: Journal
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Publ. Info: 236 GRAYS INN RD, FLOOR 6, LONDON WC1X 8HL, ENGLAND : BIOMED CENTRAL LTD
Pages: - Volume / Issue: 8 Sequence Number: 2 Start / End Page: - Identifier: ISSN: 1745-6150