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  Carbon isotope effects on the fructose-1,6-bisphosphate aldolase reaction, origin for non-statistical 13C distributions in carbohydrates

Gleixner, G., & Schmidt, H.-L. (1997). Carbon isotope effects on the fructose-1,6-bisphosphate aldolase reaction, origin for non-statistical 13C distributions in carbohydrates. The Journal of Biological Chemistry, 272(9), 5382-5387. doi:10.1074/jbc.272.9.5382.

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BGC0014.pdf (Publisher version), 246KB
 
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http://dx.doi.org/10.1074/jbc.272.9.5382 (Publisher version)
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Gleixner, G.1, Author           
Schmidt, H.-L., Author
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Free keywords: Fructose bisphosphate aldolase; rabbit muscle aldolase; fractionation; discrimination; dependence; mechanism; products; glucose; acid; food
 Abstract: The kinetic and equilibrium isotope effects on the fructose- 1,6-bisphosphate aldolase reaction have been determined using the rabbit muscle enzyme, The natural C-13 abundance for both atoms participating in the bond splitting were measured in position C-1 of dihydroxyacetone phosphate and glyceraldehyde 3-P after irreversible conversion to glycerol-3-P and 3- phosphoglycerate, respectively, and chemical degradation, The carbon isotope effects were determined comparing the C-13 content of the corresponding positions after partial and complete turnover, and after complete equilibration of the reactants. (13)(V-max/K-m) on C-3 was 1.016 +/- 0.007 and 0.997 +/- 0.009 on position C-4, and the equilibrium isotope effects K-12/K-13 on these positions were 1.0036 +/- 0.0002 and 1.0049 +/- 0.0001. The observed kinetic isotope effect on C-3 is discussed to originate from the formation of the enamine, which comes to equilibrium before the rate determining release of glyceraldehyde 3-P from the ternary complex. The equilibrium isotope effect is seen as the reason for an earlier-found relative C-13 enrichment in position C-3 and C-4 of glucose and for varying enrichments in C-13 of carbohydrates from different compartments of cells. The kinetic isotope effect is suggested to cause C-13 discriminations in the C-3 pool in context with the hexose formation in competition with other dihydroxyacetone phosphate turnover reactions.

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 Dates: 1997
 Publication Status: Issued
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 Identifiers: Other: BGC0014
DOI: 10.1074/jbc.272.9.5382
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Title: The Journal of Biological Chemistry
  Other : JBC
Source Genre: Journal
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Publ. Info: Baltimore, etc. : American Society for Biochemistry and Molecular Biology [etc.]
Pages: - Volume / Issue: 272 (9) Sequence Number: - Start / End Page: 5382 - 5387 Identifier: ISSN: 0021-9258
CoNE: https://pure.mpg.de/cone/journals/resource/954925410826_1