English
 
Help Privacy Policy Disclaimer
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  Horizon-Specific Bacterial Community Composition of German Grassland Soils, as Revealed by Pyrosequencing-Based Analysis of 16S rRNA Genes

Will, C., Thurmer, A., Wollherr, A., Nacke, H., Herold, N., Schrumpf, M., et al. (2010). Horizon-Specific Bacterial Community Composition of German Grassland Soils, as Revealed by Pyrosequencing-Based Analysis of 16S rRNA Genes. Applied and Environmental Microbiology, 76(20), 6751-6759. doi:10.1128/aem.01063-10.

Item is

Files

show Files
hide Files
:
BGC1432.pdf (Publisher version), 686KB
 
File Permalink:
-
Name:
BGC1432.pdf
Description:
-
OA-Status:
Visibility:
Restricted (Max Planck Institute for Biogeochemistry, MJBK; )
MIME-Type / Checksum:
application/octet-stream
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-

Locators

show
hide
Locator:
http://dx.doi.org/10.1128/aem.01063-10 (Publisher version)
Description:
OA
OA-Status:
Green

Creators

show
hide
 Creators:
Will, C., Author
Thurmer, A., Author
Wollherr, A., Author
Nacke, H., Author
Herold, N.1, Author           
Schrumpf, M.1, Author           
Gutknecht, J., Author
Wubet, T., Author
Buscot, F., Author
Daniel, R., Author
Affiliations:
1Soil and Ecosystem Processes, Dr. M. Schrumpf, Department Biogeochemical Processes, Prof. S. E. Trumbore, Max Planck Institute for Biogeochemistry, Max Planck Society, ou_1497776              

Content

show
hide
Free keywords: microbial diversity rare biosphere biomass subsurface populations rhizobium richness length management sequences
 Abstract: The diversity of bacteria in soil is enormous, and soil bacterial communities can vary greatly in structure. Here, we employed a pyrosequencing-based analysis of the V2-V3 16S rRNA gene region to characterize the overall and horizon-specific (A and B horizons) bacterial community compositions in nine grassland soils, which covered three different land use types. The entire data set comprised 752,838 sequences, 600,544 of which could be classified below the domain level. The average number of sequences per horizon was 41,824. The dominant taxonomic groups present in all samples and horizons were the Acidobacteria, Betaproteobacteria, Actinobacteria, Gammaproteobacteria, Alphaproteobacteria, Deltaproteobacteria, Chloroflexi, Firmicutes, and Bacteroidetes. Despite these overarching dominant taxa, the abundance, diversity, and composition of bacterial communities were horizon specific. In almost all cases, the estimated bacterial diversity (H') was higher in the A horizons than in the corresponding B horizons. In addition, the H' was positively correlated with the organic carbon content, the total nitrogen content, and the C-to-N ratio, which decreased with soil depth. It appeared that lower land use intensity results in higher bacterial diversity. The majority of sequences affiliated with the Actinobacteria, Bacteroidetes, Cyanobacteria, Fibrobacteres, Firmicutes, Spirochaetes, Verrucomicrobia, Alphaproteobacteria, Betaproteobacteria, and Gammaproteobacteria were derived from A horizons, whereas the majority of the sequences related to Acidobacteria, Chloroflexi, Gemmatimonadetes, Nitrospira, TM7, and WS3 originated from B horizons. The distribution of some bacterial phylogenetic groups and subgroups in the different horizons correlated with soil properties such as organic carbon content, total nitrogen content, or microbial biomass.

Details

show
hide
Language(s): eng - English
 Dates: 2010
 Publication Status: Issued
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Identifiers: DOI: 10.1128/aem.01063-10
ISI: ://000282595100006
Other: BGC1432
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: Applied and Environmental Microbiology
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: American Society for Microbiology (ASM)
Pages: - Volume / Issue: 76 (20) Sequence Number: - Start / End Page: 6751 - 6759 Identifier: CoNE: https://pure.mpg.de/cone/journals/resource/954927519600
ISSN: 0099-2240