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  Characterization of the role of ribonucleases in Salmonella small RNA decay.

Viegas, S. C., Pfeiffer, V., Sittka, A., Silva, I. J., Vogel, J., & Arraiano, C. M. (2007). Characterization of the role of ribonucleases in Salmonella small RNA decay. Nucleic Acids Research, 35(22), 7651-7664. doi:10.1093/nar/gkm916.

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Nucl_Acids_Res_2007_35_7651.pdf (Verlagsversion), 5MB
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© 2007 The Author(s) This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.0/uk/) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
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Viegas, Sandra C., Autor
Pfeiffer, Verena1, Autor           
Sittka, Alexandra1, Autor           
Silva, Ines J., Autor
Vogel, Jörg1, Autor           
Arraiano, Cecilia M., Autor
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1Max-Planck Research Group RNA Biology, Max Planck Institute for Infection Biology, Max Planck Society, ou_1664150              

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 Zusammenfassung: In pathogenic bacteria, a large number of sRNAs coordinate adaptation to stress and expression of virulence genes. To better understand the turnover of regulatory sRNAs in the model pathogen, Salmonella typhimurium, we have constructed mutants for several ribonucleases (RNase E, RNase G, RNase III, PNPase) and Poly(A) Polymerase I. The expression profiles of four sRNAs conserved among many enterobacteria, CsrB, CsrC, MicA and SraL, were analysed and the processing and stability of these sRNAs was studied in the constructed strains. The degradosome was a common feature involved in the turnover of these four sRNAs. PAPI-mediated polyadenylation was the major factor governing SraL degradation. RNase III was revealed to strongly affect MicA decay. PNPase was shown to be important in the decay of these four sRNAs. The stability of CsrB and CsrC seemed to be independent of the RNA chaperone, Hfq, whereas the decay of SraL and MicA was Hfq-dependent. Taken together, the results of this study provide initial insight into the mechanisms of sRNA decay in Salmonella, and indicate specific contributions of the RNA decay machinery components to the turnover of individual sRNAs.

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Sprache(n): eng - English
 Datum: 2007-11-03
 Publikationsstatus: Erschienen
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 Identifikatoren: eDoc: 331484
DOI: 10.1093/nar/gkm916
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Titel: Nucleic Acids Research
Genre der Quelle: Zeitschrift
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Seiten: - Band / Heft: 35 (22) Artikelnummer: - Start- / Endseite: 7651 - 7664 Identifikator: ISSN: 0301-5610