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  Differential expression of Aspergillus fumigatus protein in response to treatment with a novel antifungal compound, diethyl 4-(4-methoxyphenyl)-2,6-dimethyl-1,4-dihydropyridin-3,5-dicarboxylate.

Chhillar, A. K., Yadav, V., Kumar, A., Kumar, M., Parmar, V. S., Prasad, A., et al. (2009). Differential expression of Aspergillus fumigatus protein in response to treatment with a novel antifungal compound, diethyl 4-(4-methoxyphenyl)-2,6-dimethyl-1,4-dihydropyridin-3,5-dicarboxylate. Mycoses, 52(3), 223-227. doi:10.1111/j.1439-0507.2008.01563.x.

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Mycoses_2009_52_223.pdf (Verlagsversion), 108KB
 
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 Urheber:
Chhillar, A. K., Autor
Yadav, Vibha1, Autor           
Kumar, A., Autor
Kumar, M., Autor
Parmar, V. S., Autor
Prasad, A., Autor
Sharma, G. L., Autor
Affiliations:
1Department of Molecular Biology, Max Planck Institute for Infection Biology, Max Planck Society, ou_1664147              

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Schlagwörter: Aspergillus fumigatus • antifungal compound • differential protein expression • probable retroelement pol polyprotein • elongation factor G
 Zusammenfassung: A dihydropyridine derivative, diethyl 4-(4-methoxyphenyl)-2,6-dimethyl-1,4-dihydropyridin-3,5-dicarboxylate (2e), having potent antifungal activity against pathogenic species of Aspergillus was investigated for its possible molecular mechanism of action. The SDS-PAGE coupled with nano-high-performance liquid chromatography–tandem mass spectrometry was used directly to assess both absolute abundance and differential expression of proteins in the secretory phases of Aspergillus fumigatus under the influence of 2e. It was observed that the compound inhibited the expression of two proteins of 60.99 and 79.77 kDa. Both of these secretory proteins that were inhibited by 2e, were analysed further by matrix assisted laser desorption ionization time-of-flight mass spectrometry. The 60.99- and 79.77-kDa proteins were identified as probable retroelement pol polyprotein and elongation factor G respectively. These targeted proteins could be the products of potentially virulence-related genes of A. fumigatus which may unravel the mode of action of 2e and pathobiology of A. fumigatus.

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Sprache(n): eng - English
 Datum: 2009
 Publikationsstatus: Erschienen
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 Art der Begutachtung: -
 Identifikatoren: eDoc: 460519
DOI: 10.1111/j.1439-0507.2008.01563.x
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Titel: Mycoses
Genre der Quelle: Zeitschrift
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Ort, Verlag, Ausgabe: -
Seiten: - Band / Heft: 52 (3) Artikelnummer: - Start- / Endseite: 223 - 227 Identifikator: ISSN: 0933-7407