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  Comparative Proteomics of Two Life Cycle Stages of Stable Isotope-labeled Trypanosoma brucei Reveals Novel Components of the Parasite's Host Adaptation Machinery

Butter, F., Bucerius, F., Michel, M., Cicova, Z., Mann, M., & Janzen, C. J. (2013). Comparative Proteomics of Two Life Cycle Stages of Stable Isotope-labeled Trypanosoma brucei Reveals Novel Components of the Parasite's Host Adaptation Machinery. MOLECULAR & CELLULAR PROTEOMICS, 12(1), 172-179. doi:10.1074/mcp.M112.019224.

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 Urheber:
Butter, Falk1, Autor           
Bucerius, Ferdinand2, Autor
Michel, Margaux2, Autor
Cicova, Zdenka2, Autor
Mann, Matthias1, Autor           
Janzen, Christian J.2, Autor
Affiliations:
1Mann, Matthias / Proteomics and Signal Transduction, Max Planck Institute of Biochemistry, Max Planck Society, ou_1565159              
2external, ou_persistent22              

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Schlagwörter: BLOOD-STREAM FORMS; QUANTITATIVE PROTEOMICS; PROTEIN QUANTIFICATION; EXPRESSION; DIFFERENTIATION; IDENTIFICATION; TRANSFORMATION; TRANSCRIPTOME; CULTIVATION; METABOLISM
 Zusammenfassung: Trypanosoma brucei developed a sophisticated life cycle to adapt to different host environments. Although developmental differentiation of T. brucei has been the topic of intensive research for decades, the mechanisms responsible for adaptation to different host environments are not well understood. We developed stable isotope labeling by amino acids in cell culture in trypanosomes to compare the proteomes of two different life cycle stages. Quantitative comparison of 4364 protein groups identified many proteins previously not known to be stage-specifically expressed. The identification of stage-specific proteins helps to understand how parasites adapt to different hosts and provides new insights into differences in metabolism, gene regulation, and cell architecture. A DEAD-box RNA helicase, which is highly up-regulated in the bloodstream form of this parasite and which is essential for viability and proper cell cycle progression in this stage is described as an example. Molecular & Cellular Proteomics 12: 10.1074/mcp.M112.019224, 172-179, 2013.

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Sprache(n): eng - English
 Datum: 2013-01
 Publikationsstatus: Erschienen
 Seiten: 8
 Ort, Verlag, Ausgabe: -
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 Art der Begutachtung: Expertenbegutachtung
 Identifikatoren: ISI: 000313557600013
DOI: 10.1074/mcp.M112.019224
 Art des Abschluß: -

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Titel: MOLECULAR & CELLULAR PROTEOMICS
Genre der Quelle: Zeitschrift
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Ort, Verlag, Ausgabe: 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3996 USA : AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC
Seiten: - Band / Heft: 12 (1) Artikelnummer: - Start- / Endseite: 172 - 179 Identifikator: ISSN: 1535-9476