ausblenden:
Schlagwörter:
RETROGRADE TRANSPORT; VACUOLAR HYDROLASES; CA2+ HOMEOSTASIS; COATED
VESICLES; KDEL RECEPTOR; PROTEINS; YEAST; PATHWAY; TGN; COMPARTMENT
Zusammenfassung:
Ca2+ import into the lumen of the trans-Golgi network (TGN) by the secretory pathway calcium ATPase1 (SPCA1) is required for the sorting of secretory cargo. How is Ca2+ retained in the lumen of the Golgi, and what is its role in cargo sorting? We show here that a soluble, lumenal Golgi resident protein, Cab45, is required for SPCA1-dependent Ca2+ import into the TGN; it binds secretory cargo in a Ca2+-dependent reaction and is required for its sorting at the TGN.
