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  Drosophila Psidin Regulates Olfactory Neuron Number and Axon Targeting through Two Distinct Molecular Mechanisms

Stephan, D., Sanchez-Soriano, N., Loschek, L. F., Gerhards, R., Gutmann, S., Storchova, Z., et al. (2012). Drosophila Psidin Regulates Olfactory Neuron Number and Axon Targeting through Two Distinct Molecular Mechanisms. JOURNAL OF NEUROSCIENCE, 32(46), 16080-16094. doi:10.1523/JNEUROSCI.3116-12.2012.

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Stephan, Daniel1, Autor           
Sanchez-Soriano, Natalia2, Autor
Loschek, Laura F.1, Autor           
Gerhards, Ramona1, Autor           
Gutmann, Susanne3, Autor           
Storchova, Zuzana3, Autor           
Prokop, Andreas2, Autor
Kadow, Ilona C. Grunwald1, Autor           
Affiliations:
1Max Planck Research Group: Sensory Neurogenetics / Grunwald-Kadow, MPI of Neurobiology, Max Planck Society, ou_1113556              
2external, ou_persistent22              
3Storchova, Zuzana / Maintenance of Genome Stability, Max Planck Institute of Biochemistry, Max Planck Society, ou_1565171              

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Schlagwörter: GROWTH CONE; PROTEINS; GUIDANCE; DYNAMICS; TROPOMYOSIN; MOTILITY; SYSTEM; RHO
 Zusammenfassung: The formation of neuronal circuits is a key process of development, laying foundations for behavior. The cellular mechanisms regulating circuit development are not fully understood. Here, we reveal Psidin as an intracellular regulator of Drosophila olfactory system formation. We show that Psidin is required in several classes of olfactory receptor neurons (ORNs) for survival and subsequently for axon guidance. During axon guidance, Psidin functions as an actin regulator and antagonist of Tropomyosin. Accordingly, Psidin-deficient primary neurons in culture display growth cones with significantly smaller lamellipodia. This lamellipodial phenotype, as well as the mistargeting defects in vivo, is suppressed by parallel removal of Tropomyosin. In contrast, Psidin functions as the noncatalytic subunit of the N-acetyltransferase complex B (NatB) to maintain the number of ORNs. Psidin physically binds the catalytic NatB subunit CG14222 (dNAA20) and functionally interacts with it in vivo. We define the dNAA20 interaction domain within Psidin and identify a conserved serine as a candidate for phosphorylation-mediated regulation of NatB complex formation. A phosphomimetic mutation of this serine showed severely reduced binding to dNAA20 in vitro. In vivo, it fully rescued the targeting defect but not the reduction in neuron numbers. In addition, we show that a different amino acid point mutation shows exactly the opposite effect by rescuing only the cell number but not the axon targeting defect. Together, our data suggest that Psidin plays two independent developmental roles via the acquisition of separate signaling pathways, both of which contribute to the formation of olfactory circuits.

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Sprache(n): eng - English
 Datum: 2012-11-14
 Publikationsstatus: Erschienen
 Seiten: 15
 Ort, Verlag, Ausgabe: -
 Inhaltsverzeichnis: -
 Art der Begutachtung: Expertenbegutachtung
 Identifikatoren: ISI: 000311091000003
DOI: 10.1523/JNEUROSCI.3116-12.2012
 Art des Abschluß: -

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Titel: JOURNAL OF NEUROSCIENCE
Genre der Quelle: Zeitschrift
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Ort, Verlag, Ausgabe: 11 DUPONT CIRCLE, NW, STE 500, WASHINGTON, DC 20036 USA : SOC NEUROSCIENCE
Seiten: - Band / Heft: 32 (46) Artikelnummer: - Start- / Endseite: 16080 - 16094 Identifikator: ISSN: 0270-6474