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  Transcriptional activities of the zinc finger protein Zac are differentially controlled by DNA binding

Hoffmann, A., Ciani, E., Boeckardt, J., Holsboer, F., Journot, L., & Spengler, D. (2003). Transcriptional activities of the zinc finger protein Zac are differentially controlled by DNA binding. Molecular and Cellular Biology, 23(3), 988-1003.

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Datensatz-Permalink: https://hdl.handle.net/11858/00-001M-0000-000E-9F43-8 Versions-Permalink: https://hdl.handle.net/11858/00-001M-0000-000E-9F44-6
Genre: Zeitschriftenartikel
Alternativer Titel : Mol. Cell. Biol.

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 Urheber:
Hoffmann, A, Autor
Ciani, E, Autor
Boeckardt, J, Autor
Holsboer, F1, Autor
Journot, L, Autor
Spengler, D1, Autor
Affiliations:
1Max Planck Institute of Psychiatry, Max Planck Society, ou_1607137              

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Schlagwörter: -
 Zusammenfassung: Zac encodes a zinc finger protein that promotes apoptosis and cell cycle arrest and is maternally imprinted. Here, we show that Zac contains transactivation and repressor activities and that these transcriptional activities are differentially controlled by DNA binding. Zac transactivation mapped to two distinct domains. One of these contained multiple repeats of the peptide PLE, which behaved as an autonomous activation unit. More importantly, we identified two related high-affinity DNA-binding sites which were differentially bound by seven Zac C2H2 zinc fingers. Zac bound as a monomer through zinc fingers 6 and 7 to the palindromic DNA element to confer transactivation. In contrast, binding as a monomer to one half- site of the repeat element turned Zac into a repressor. Conversely, Zac dimerization at properly spaced direct and reverse repeat elements enabled transactivation, which strictly correlated with DNA-dependent and -independent contacts of key residues within the recognition helix of zinc finger 7. The later ones support specific functional connections between Zac DNA binding and transcriptional-regulatory surfaces. Both classes of DNA elements were identified in a new Zac target gene and confirmed that the zinc fingers communicate with the transactivation function. Together, our data demonstrate a role for Zac as a transcription factor in addition to its role as coactivator for nuclear receptors and p5

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Sprache(n): eng - English
 Datum: 2003-02
 Publikationsstatus: Erschienen
 Seiten: -
 Ort, Verlag, Ausgabe: -
 Inhaltsverzeichnis: -
 Art der Begutachtung: Expertenbegutachtung
 Identifikatoren: eDoc: 4001
ISI: 000180673600021
 Art des Abschluß: -

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Titel: Molecular and Cellular Biology
  Alternativer Titel : Mol. Cell. Biol.
Genre der Quelle: Zeitschrift
 Urheber:
Affiliations:
Ort, Verlag, Ausgabe: -
Seiten: - Band / Heft: 23 (3) Artikelnummer: - Start- / Endseite: 988 - 1003 Identifikator: ISSN: 0270-7306