Deutsch
 
Hilfe Datenschutzhinweis Impressum
  DetailsucheBrowse

Datensatz

DATENSATZ AKTIONENEXPORT
  Modulation of Ligand-Gated Ion Channels as a Novel Pharmacological Principle

Nothdurfter, C., Tanasic, S., Rammes, G., & Rupprecht, R. (2011). Modulation of Ligand-Gated Ion Channels as a Novel Pharmacological Principle. Pharmacopsychiatry, 44(Suppl. 1), S27-S34.

Item is

Externe Referenzen

einblenden:

Urheber

einblenden:
ausblenden:
 Urheber:
Nothdurfter, C.1, Autor           
Tanasic, S.1, Autor           
Rammes, G.2, Autor           
Rupprecht, R.1, Autor           
Affiliations:
1Rainer Rupprecht (Max-Planck Fellow), Max Planck Institute of Psychiatry, Max Planck Society, ou_1607140              
2AG Eder, Matthias, Florian Holsboer (Direktor), Max Planck Institute of Psychiatry, Max Planck Society, ou_1607146              

Inhalt

einblenden:
ausblenden:
Schlagwörter: -
 Zusammenfassung: The present study investigated the functional antagonism of different antidepressants on 5-HT3 receptor function and the role of lipid rafts for these modulatory effects. Electrophysiological recordings of 5-HT evoked cation currents were recorded with N1E-115 and HEK-5-HT3A cells and hippocampal neurons. The characterization of the antagonism of antidepressants was made by the displacement of [H-3]GR65630 binding. For membrane fractionation, sucrose density gradient centrifugation was used. Gradient fractions were assayed for antidepressant concentrations by HPLC; 5-HT3 receptor membrane distribution was determined by Western blot. Colocalization experiments were performed by means of immunocytochemistry. Most antidepressants acted as non-competitive antagonists at the 5-HT3 receptor. Moreover, some of these compounds were enriched within lipid rafts. Cholesterol depletion impaired lipid raft integrity thereby affecting 5-HT3 receptor function, whereas the antagonistic effects of antidepressants were not altered. In conclusion, most antidepressants directly antagonize 5-HT3 receptor activity. 5-HT3 receptor function per se appears to depend on lipid raft integrity, which is, however, not a prerequisite for the modulatory potency of antidepressants at this receptor.

Details

einblenden:
ausblenden:
Sprache(n): eng - English
 Datum: 2011-05
 Publikationsstatus: Erschienen
 Seiten: -
 Ort, Verlag, Ausgabe: -
 Inhaltsverzeichnis: -
 Art der Begutachtung: -
 Identifikatoren: eDoc: 563801
ISI: 000290416000005
 Art des Abschluß: -

Veranstaltung

einblenden:

Entscheidung

einblenden:

Projektinformation

einblenden:

Quelle 1

einblenden:
ausblenden:
Titel: Pharmacopsychiatry
Genre der Quelle: Zeitschrift
 Urheber:
Affiliations:
Ort, Verlag, Ausgabe: -
Seiten: - Band / Heft: 44 (Suppl. 1) Artikelnummer: - Start- / Endseite: S27 - S34 Identifikator: ISSN: 0176-3679