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  Visualization of Endothelial Actin Cytoskeleton in the Mouse Retina

Fraccaroli, A., Franco, C. A., Rognoni, E., Filipa, N., Rehberg, M., Aszodi, A., et al. (2012). Visualization of Endothelial Actin Cytoskeleton in the Mouse Retina. PLOS ONE, 7(10): e47488. doi:10.1371/journal.pone.0047488.

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Fraccaroli, Alessia1, Autor
Franco, Claudio A.1, Autor
Rognoni, Emanuel2, Autor           
Filipa, Neto1, Autor
Rehberg, Markus1, Autor
Aszodi, Attila1, Autor           
Wedlich-Söldner, Roland3, Autor           
Pohl, Ulrich1, Autor
Gerhardt, Holger1, Autor
Montanez, Eloi1, Autor           
Affiliations:
1external, ou_persistent22              
2Fässler, Reinhard / Molecular Medicine, Max Planck Institute of Biochemistry, Max Planck Society, ou_1565147              
3Wedlich-Söldner, Roland / Cellular Dynamics and Cell Patterning, Max Planck Institute of Biochemistry, Max Planck Society, ou_1565174              

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Schlagwörter: F-ACTIN; IN-VIVO; ANGIOGENESIS; VASCULATURE; FILOPODIA; LIFEACT; CELLS; MICE
 Zusammenfassung: Angiogenesis requires coordinated changes in cell shape of endothelial cells (ECs), orchestrated by the actin cytoskeleton. The mechanisms that regulate this rearrangement in vivo are poorly understood - largely because of the difficulty to visualize filamentous actin (F-actin) structures with sufficient resolution. Here, we use transgenic mice expressing Lifeact-EGFP to visualize F-actin in ECs. We show that in the retina, Lifeact-EGFP expression is largely restricted to ECs allowing detailed visualization of F-actin in ECs in situ. Lifeact-EGFP labels actin associated with cell-cell junctions, apical and basal membranes and highlights actin-based structures such as filopodia and stress fiber-like cytoplasmic bundles. We also show that in the skin and the skeletal muscle, Lifeact-EGFP is highly expressed in vascular mural cells (vMCs), enabling vMC imaging. In summary, our results indicate that the Lifeact-EGFP transgenic mouse in combination with the postnatal retinal angiogenic model constitutes an excellent system for vascular cell biology research. Our approach is ideally suited to address structural and mechanistic details of angiogenic processes, such as endothelial tip cell migration and fusion, EC polarization or lumen formation.

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Sprache(n): eng - English
 Datum: 2012-10-24
 Publikationsstatus: Online veröffentlicht
 Seiten: 9
 Ort, Verlag, Ausgabe: -
 Inhaltsverzeichnis: -
 Art der Begutachtung: Expertenbegutachtung
 Identifikatoren: ISI: 000310310200043
DOI: 10.1371/journal.pone.0047488
 Art des Abschluß: -

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Titel: PLOS ONE
Genre der Quelle: Zeitschrift
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Ort, Verlag, Ausgabe: 1160 BATTERY STREET, STE 100, SAN FRANCISCO, CA 94111 USA : PUBLIC LIBRARY SCIENCE
Seiten: - Band / Heft: 7 (10) Artikelnummer: e47488 Start- / Endseite: - Identifikator: ISSN: 1932-6203