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  Formation of 70S ribosomes: large activation energy is required for the adaptation of exclusively the small ribosomal subunit

Blaha, G., Burkhardt, N., & Nierhaus, K. H. (2002). Formation of 70S ribosomes: large activation energy is required for the adaptation of exclusively the small ribosomal subunit. Biophysical Chemistry, 96(2-3), 153-161.

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Blaha, Gregor1, Autor           
Burkhardt, Nils2, Autor
Nierhaus, Knud H.1, Autor           
Affiliations:
1Ribosomes, Max Planck Institute for Molecular Genetics, Max Planck Society, ou_1433558              
2Max Planck Society, ou_persistent13              

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Schlagwörter: Protein synthesis; Ribosome; Activation energy; Association of ribosomal subunits
 Zusammenfassung: Association of ribosomal subunits is an essential reaction during the initiation phase of protein synthesis. Optimal conditions for 70S formation in vitro were determined to 20 mM Mg2+ and 30 mM K+. Under these conditions, the association reaction proceeds with first order kinetics, suggesting a conformational change to be the rate-limiting step. 70S formation separates into two sub-reactions, the adaptation of the ribosomal subunits to the association conditions and the association step itself. The activation energy of the process was determined to 78 kJ/mol and revealed to be required exclusively for the adaptation of the small subunit, rather than the large subunit or the association step. The presence of mRNA [poly(U)] together with cognate AcPhe-tRNA, accelerates the association rate significantly, forming a well-defined 70S peak in sucrose gradient profiles. mRNA alone provokes an equivalent acceleration, however, the resulting 70S couple impresses as an ill-defined, broad peak, probably indicating the readiness of the ribosome for tRNA binding, upon which the ribosome flips into a defined state.

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Sprache(n): eng - English
 Datum: 2002-02-01
 Publikationsstatus: Erschienen
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 Ort, Verlag, Ausgabe: -
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 Identifikatoren: eDoc: 24717
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Titel: Biophysical Chemistry
Genre der Quelle: Zeitschrift
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Ort, Verlag, Ausgabe: -
Seiten: - Band / Heft: 96 (2-3) Artikelnummer: - Start- / Endseite: 153 - 161 Identifikator: -