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  Genetic analysis of the mouse brain proteome

Klose, J., Nock, C., Herrmann, M., Stühler, K., Marcus, K., Blüggel, M., et al. (2002). Genetic analysis of the mouse brain proteome. Nature Genetics, 30(4), 385-393.

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 Creators:
Klose, Joachim, Author
Nock, Christina1, Author
Herrmann, Marion, Author
Stühler, Kai, Author
Marcus, Katrin, Author
Blüggel, Martin, Author
Krause, Eberhard, Author
Schalkwyk, Leonard C., Author
Rastan, Sohaila, Author
Brown, Steve D.M., Author
Büssow, Konrad2, Author           
Himmelbauer, Heinz2, Author           
Lehrach, Hans2, Author           
Affiliations:
1Max Planck Society, ou_persistent13              
2Dept. of Vertebrate Genomics (Head: Hans Lehrach), Max Planck Institute for Molecular Genetics, Max Planck Society, ou_1433550              

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 Abstract: Proteome analysis is a fundamental step in systematic functional genomics. Here we have resolved 8,767 proteins from the mouse brain proteome by large-gel two-dimensional electrophoresis. We detected 1,324 polymorphic proteins from the European collaborative interspecific backcross. Of these, we mapped 665 proteins genetically and identified 466 proteins by mass spectrometry. Qualitatively polymorphic proteins, to 96%, reflect changes in conformation and/or mass. Quantitatively polymorphic proteins show a high frequency (73%) of allele-specific transmission in codominant heterozygotes. Variations in protein isoforms and protein quantity often mapped to chromosomal positions different from that of the structural gene, indicating that single proteins may act as polygenic traits. Genetic analysis of proteomes may detect the types of polymorphism that are most relevant in disease-association studies.

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Language(s): eng - English
 Dates: 2002-03-25
 Publication Status: Issued
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Identifiers: eDoc: 25849
 Degree: -

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Title: Nature Genetics
Source Genre: Journal
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Pages: - Volume / Issue: 30 (4) Sequence Number: - Start / End Page: 385 - 393 Identifier: -