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  TraG-Like Proteins of DNA Transfer Systems and of the Helicobacter pylori Type IV Secretion System: Inner Membrane Gate for Exported Substrates?

Schröder, G., Krause, S., Zechner, E. L., Traxler, B., Yeo, H.-J., Lurz, R., et al. (2002). TraG-Like Proteins of DNA Transfer Systems and of the Helicobacter pylori Type IV Secretion System: Inner Membrane Gate for Exported Substrates? Journal of Bacteriology, 184(10), 2767-2779.

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Item Permalink: https://hdl.handle.net/11858/00-001M-0000-0010-8C04-1 Version Permalink: https://hdl.handle.net/11858/00-001M-0000-0010-8C05-0
Genre: Journal Article
Alternative Title : J Bacteriol

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 Creators:
Schröder, Gunnar1, Author
Krause, Sabine1, Author
Zechner, Ellen L., Author
Traxler, Beth, Author
Yeo, Hye-Jeong, Author
Lurz, Rudi1, Author
Waksman, Gabriel, Author
Lanka, Erich1, Author
Affiliations:
1Max Planck Society, ou_persistent13              

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 Abstract: TraG-like proteins are potential NTP hydrolases (NTPases) that are essential for DNA transfer in bacterial conjugation. They are thought to mediate interactions between the DNA-processing (Dtr) and the mating pair formation (Mpf) systems. TraG-like proteins also function as essential components of type IV secretion systems of several bacterial pathogens such as Helicobacter pylori. Here we present the biochemical characterization of three members of the family of TraG-like proteins, TraG (RP4), TraD (F), and HP0524 (H. pylori). These proteins were found to have a pronounced tendency to form oligomers and were shown to bind DNA without sequence specificity. Standard NTPase assays indicated that these TraG-like proteins do not possess postulated NTP-hydrolyzing activity. Surface plasmon resonance was used to demonstrate an interaction between TraG and relaxase TraI of RP4. Topology analysis of TraG revealed that TraG is a transmembrane protein with cytosolic N and C termini and a short periplasmic domain close to the N terminus. We predict that multimeric inner membrane protein TraG forms a pore. A model suggesting that the relaxosome binds to the TraG pore via TraG-DNA and TraG-TraI interactions is presented.

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Language(s): eng - English
 Dates: 2002-05
 Publication Status: Issued
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Identifiers: eDoc: 24581
 Degree: -

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Title: Journal of Bacteriology
  Alternative Title : J Bacteriol
Source Genre: Journal
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Publ. Info: -
Pages: - Volume / Issue: 184 (10) Sequence Number: - Start / End Page: 2767 - 2779 Identifier: -