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  The complete nucleotide sequence and environmental distribution of the cryptic, conjugative, broad-host-range plasmid pIPO2 isolated from bacteria of the wheat rhizosphere

Tauch, A., Schneiker, S., Selbitschka, W., Pühler, A., van Overbeek, L. S., Smalla, K., et al. (2002). The complete nucleotide sequence and environmental distribution of the cryptic, conjugative, broad-host-range plasmid pIPO2 isolated from bacteria of the wheat rhizosphere. Microbiology, 148(Pt 6), 1637-1653.

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Item Permalink: https://hdl.handle.net/11858/00-001M-0000-0010-8BF4-4 Version Permalink: https://hdl.handle.net/11858/00-001M-0000-0010-8BF5-2
Genre: Journal Article
Alternative Title : Microbiol

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 Creators:
Tauch, Andreas, Author
Schneiker, Susanne, Author
Selbitschka, Werner, Author
Pühler, Alfred, Author
van Overbeek, Leo S., Author
Smalla, Kornelia, Author
Thomas, Christopher M., Author
Bailey, Mark J., Author
Forney, Larry J., Author
Weightman, Andrew, Author
Ceglowski, Piotr, Author
Pembroke, Tony, Author
Tietze, Erhard, Author
Schröder, Gunnar1, Author
Lanka, Erich1, Author
van Elsas, Jan Dirk, Author
Affiliations:
1Max Planck Society, ou_persistent13              

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Free keywords: bacterial genome, exogenous plasmid isolation, plasmid stability, conjugative transfer, horizontal gene spread
 Abstract: Plasmid pIPO2 is a cryptic, conjugative, broad-host-range plasmid isolated from the wheat rhizosphere. It efficiently self-transfers between , ß and Proteobacteria and has a mobilizing/retromobilizing capacity for IncQ plasmids. The complete nucleotide sequence of pIPO2 is presented on the basis of its mini-Tn5::luxABtet-tagged derivative, pIPO2T. The pIPO2 sequence is 39815 bp long and contains at least 43 complete ORFs. Apart from a suite of ORFs with unknown function, all of the genes carried on pIPO2 are predicted to be involved in plasmid replication, maintenance and conjugative transfer. The overall organization of these genes is different from previously described plasmids, but is similar to the genetic organization seen in pSB102, a conjugative plasmid recently isolated from the bacterial community of the alfalfa rhizosphere. The putative conjugative transfer region of pIPO2 covers 23 kb and contains the genes required for DNA processing (Dtr) and mating pair formation (Mpf). The organization of these transfer genes in pIPO2 is highly similar to the genetic organization seen in the environmental plasmid pSB102 and in pXF51 from the plant pathogen Xylella fastidiosa. Plasmids pSB102 and pXF51 have recently been proposed to form a new family of environmental broad-host-range plasmids. Here it is suggested that pIPO2 is a new member of this family. The proposed Mpf system of pIPO2 shares high amino acid sequence similarity with equivalent VirB proteins from the type IV secretion system of Brucella spp. Sequence information was used to design primers specific for the detection of pIPO2. Environmental DNA from a range of diverse habitats was screened by PCR with these primers. Consistently positive signals for the presence of pIPO2 were obtained from a range of soil-related habitats, including the rhizospheres of young wheat plants, of field-grown oats and of grass (all gramineous plants), as well as from the rhizosphere of tomato plants. These data add to the growing evidence that plasmids carry advantageous genes with as yet undefined functions in plant-associated communities.

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Language(s): eng - English
 Dates: 2002-06
 Publication Status: Issued
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Identifiers: eDoc: 24576
 Degree: -

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Title: Microbiology
  Alternative Title : Microbiol
Source Genre: Journal
 Creator(s):
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Publ. Info: -
Pages: - Volume / Issue: 148 (Pt 6) Sequence Number: - Start / End Page: 1637 - 1653 Identifier: -