de.mpg.escidoc.pubman.appbase.FacesBean
Deutsch
 
Hilfe Wegweiser Datenschutzhinweis Impressum Kontakt
  DetailsucheBrowse

Datensatz

 
 
 
 
DownloadE-Mail
  Biophysical study of a molecular intermediate preceding collapse of tight couple and Kaltschmidt–Wittmann ribosomes

Bonincontro, A., Nierhaus, K. H., Ortore, M. G., & Risuleo, G. (2002). Biophysical study of a molecular intermediate preceding collapse of tight couple and Kaltschmidt–Wittmann ribosomes. FEBS Letters, 525(1-3), 111-115.

Item is

Basisdaten

einblenden: ausblenden:
Datensatz-Permalink: http://hdl.handle.net/11858/00-001M-0000-0010-8BD4-C Versions-Permalink: http://hdl.handle.net/11858/00-001M-0000-0010-8BD5-A
Genre: Zeitschriftenartikel

Externe Referenzen

einblenden:

Urheber

einblenden:
ausblenden:
 Urheber:
Bonincontro, Adalberto, Autor
Nierhaus, Knud H.1, Autor              
Ortore, Maria Grazia, Autor
Risuleo, Gianfranco, Autor
Affiliations:
1Ribosomes, Max Planck Institute for Molecular Genetics, Max Planck Society, escidoc:1433558              

Inhalt

einblenden:
ausblenden:
Schlagwörter: Ribosome; Dielectric spectroscopy; Fluorescence; Light scattering
 Zusammenfassung: In previous works we evidenced, by different biophysical approaches, two levels of structural organization in Escherichia coli ribosomal particles. Thermal treatment up to a defined and non-denaturing temperature causes demolition of only one level of structural complexity. By consequence the ribosomal particle exists in an intermediate state between the native form and the completely collapsed one. In this communication we report on a structural comparison of this intermediate state in Kaltschmidt–Wittmann (LC) and `tight couple' (TC) ribosomes. Three different biophysical approaches were adopted: dielectric spectroscopy, fluorescence and light scattering. Differential responses to thermal treatment are evidenced in the two ribosomal species. In particular TC show a more compact structure and the overall particle population is more homogeneous than LC in the native state. On the other hand, LC particles after thermal treatment undergo major alterations of geometry and/or phenomena of supra-particle aggregation.

Details

einblenden:
ausblenden:
Sprache(n): eng - Englisch
 Datum: 2002-07-20
 Publikationsstatus: Im Druck publiziert
 Seiten: -
 Ort, Verlag, Ausgabe: -
 Inhaltsverzeichnis: -
 Art der Begutachtung: -
 Identifikatoren: eDoc: 24724
 Art des Abschluß: -

Veranstaltung

einblenden:

Entscheidung

einblenden:

Projektinformation

einblenden:

Quelle 1

einblenden:
ausblenden:
Titel: FEBS Letters
Genre der Quelle: Zeitschrift
 Urheber:
Affiliations:
Ort, Verlag, Ausgabe: -
Seiten: - Band / Heft: 525 (1-3) Artikelnummer: - Start- / Endseite: 111 - 115 Identifikator: -