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  Biophysical study of a molecular intermediate preceding collapse of tight couple and Kaltschmidt–Wittmann ribosomes

Bonincontro, A., Nierhaus, K. H., Ortore, M. G., & Risuleo, G. (2002). Biophysical study of a molecular intermediate preceding collapse of tight couple and Kaltschmidt–Wittmann ribosomes. FEBS Letters, 525(1-3), 111-115.

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 Creators:
Bonincontro, Adalberto, Author
Nierhaus, Knud H.1, Author           
Ortore, Maria Grazia, Author
Risuleo, Gianfranco, Author
Affiliations:
1Ribosomes, Max Planck Institute for Molecular Genetics, Max Planck Society, ou_1433558              

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Free keywords: Ribosome; Dielectric spectroscopy; Fluorescence; Light scattering
 Abstract: In previous works we evidenced, by different biophysical approaches, two levels of structural organization in Escherichia coli ribosomal particles. Thermal treatment up to a defined and non-denaturing temperature causes demolition of only one level of structural complexity. By consequence the ribosomal particle exists in an intermediate state between the native form and the completely collapsed one. In this communication we report on a structural comparison of this intermediate state in Kaltschmidt–Wittmann (LC) and `tight couple' (TC) ribosomes. Three different biophysical approaches were adopted: dielectric spectroscopy, fluorescence and light scattering. Differential responses to thermal treatment are evidenced in the two ribosomal species. In particular TC show a more compact structure and the overall particle population is more homogeneous than LC in the native state. On the other hand, LC particles after thermal treatment undergo major alterations of geometry and/or phenomena of supra-particle aggregation.

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Language(s): eng - English
 Dates: 2002-07-20
 Publication Status: Issued
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Identifiers: eDoc: 24724
 Degree: -

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Title: FEBS Letters
Source Genre: Journal
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Pages: - Volume / Issue: 525 (1-3) Sequence Number: - Start / End Page: 111 - 115 Identifier: -