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  MALDI mass spectrometry analysis of single nucleotide polymorphisms by photocleavage and charge-tagging

Sauer, S., Lehrach, H., & Reinhardt, R. (2003). MALDI mass spectrometry analysis of single nucleotide polymorphisms by photocleavage and charge-tagging. Nucleic Acids Research, 31(11): e63.

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Genre: Zeitschriftenartikel
Alternativer Titel : Nucleic Acids Res.

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 Urheber:
Sauer, Sascha1, Autor           
Lehrach, Hans2, Autor           
Reinhardt, Richard3, Autor           
Affiliations:
1Nutrigenomics and Gene Regulation (Sascha Sauer), Independent Junior Research Groups (OWL), Max Planck Institute for Molecular Genetics, Max Planck Society, ou_1479662              
2Dept. of Vertebrate Genomics (Head: Hans Lehrach), Max Planck Institute for Molecular Genetics, Max Planck Society, ou_1433550              
3High Throughput Technologies, Max Planck Institute for Molecular Genetics, Max Planck Society, ou_1433552              

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 Zusammenfassung: High-throughput procedures are an important requirement for future large-scale genetic studies such as genotyping of single nucleotide polymorphisms (SNPs). Matrix-assisted laser desorption/ ionisation mass spectrometry (MALDI-MS) has revolutionised the analysis of biomolecules and, in particular, provides a very attractive solution for the rapid typing of DNA. The analysis of DNA by MALDI can be significantly facilitated by a procedure termed ‘charge-tagging’. We show here a novel approach for the generation of charge-tagged DNA using a photocleavable linker and its implementation in a molecular biological procedure for SNP genotyping consisting of PCR, primer extension, photocleavage and a chemical reaction prior to MALDI target preparation and analysis. The reaction sequence is amenable to liquid handling automation and requires no stringent purification procedures. We demonstrate this new method on SNPs in two genes involved in complex traits.

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Sprache(n): eng - English
 Datum: 2003-06-01
 Publikationsstatus: Erschienen
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 Ort, Verlag, Ausgabe: -
 Inhaltsverzeichnis: -
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 Identifikatoren: eDoc: 173896
ISI: 000183231000005
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Titel: Nucleic Acids Research
  Alternativer Titel : Nucleic Acids Res.
Genre der Quelle: Zeitschrift
 Urheber:
Affiliations:
Ort, Verlag, Ausgabe: -
Seiten: - Band / Heft: 31 (11) Artikelnummer: e63 Start- / Endseite: - Identifikator: ISSN: 0305-1048