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  NET1 and HFI1 genes of yeast mediate both chromosome maintenance and mitochondrial rho(-) mutagenesis

Koltovaya, N. A., Guerasimova, A. S., Tchekhouta, I. A., & Devin, A. B. (2003). NET1 and HFI1 genes of yeast mediate both chromosome maintenance and mitochondrial rho(-) mutagenesis. Yeast, 20(11), 955-971. doi:10.1002/yea.1010.

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Genre: Journal Article
Alternative Title : Yeast

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 Creators:
Koltovaya, Natalia A., Author
Guerasimova, Anna S.1, Author
Tchekhouta, I. A., Author
Devin, A. B., Author
Affiliations:
1Max Planck Society, ou_persistent13              

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Free keywords: Saccharomyces cerevisiae, rho(-) mutability of mitochondrial genome, maintenance of chromosomes and recombinant plasmids, SRM genes, NE1 gene, HFI1 gene
 Abstract: An increase in the mitochondrial rho- mutagenesis is a well-known response of yeast cells to mutations in numerous nuclear genes as well as to various kinds of stress. Despite extensive studies for several decades, the biological significance of this response is still not fully understood. The genetic approach to solving this enigma includes a study of genes that are required for the high incidence of spontaneous rho- mutants. We have obtained mutations of a few nuclear genes of that sort and found that mutations in certain genes, including CDC28, the central cell-cycle regulation gene, result in a decrease in spontaneous rho- mutability and simultaneously affect the maintenance of the yeast chromosomes and plasmids. Two more genes resembling CDC28 in this respect are identified in the present work as a result of the characterization of four new mutants. These two genes are NET1 and HFI1 which mediate important regulatory protein-protein interactions in the yeast cell. The effects of four mutations, including net1-srm and hfi1-srm, on the maintenance of the yeast mitochondrial genome, chromosomes and plasmids, as well as on the cell's sensitivity to ionizing radiation, are also described. The data presented suggest that the pleiotropic srm mutations determining coordinate changes in the fidelity of mitotic transmission of chromosomes, plasmids and mtDNA molecules identify genes that most probably operate high up in the hierarchy of the general genetic regulation of yeast.

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Language(s): eng - English
 Dates: 2003-08
 Publication Status: Issued
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Identifiers: eDoc: 174674
ISI: 000184839400003
DOI: 10.1002/yea.1010
 Degree: -

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Title: Yeast
  Alternative Title : Yeast
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: -
Pages: - Volume / Issue: 20 (11) Sequence Number: - Start / End Page: 955 - 971 Identifier: ISSN: 0749-503X