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  Telomere-independent homologue pairing and checkpoint escape of accessory ring chromosomes in male mouse meiosis

Voet, T., Liebe, B., Labaere, C., Marynen, P., & Scherthan, H. (2003). Telomere-independent homologue pairing and checkpoint escape of accessory ring chromosomes in male mouse meiosis. Journal of Cell Biology, 162(5), 795-807. doi:10.1083/jcb.200305065.

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Genre: Journal Article
Alternative Title : J. Cell Biol.

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 Creators:
Voet, Thierry, Author
Liebe, Bodo1, Author
Labaere, Charlotte, Author
Marynen, Peter, Author
Scherthan, Harry2, Author           
Affiliations:
1Max Planck Society, ou_persistent13              
2Dept. of Human Molecular Genetics (Head: Hans-Hilger Ropers), Max Planck Institute for Molecular Genetics, Max Planck Society, ou_1433549              

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Free keywords: chromosomal vector; spindle checkpoint; bouquet; synapsis; sex body
 Abstract: We analyzed transmission of a ring minichromosome (MC) through mouse spermatogenesis as a monosome and in the presence of a homologue. Mice, either monosomic or disomic for the MC, produced MC+ offspring. In the monosomic condition, most univalents underwent self-synapsis as indicated by STAG3, SCP3, and SCP1 deposition. Fluorescent in situ hybridization and three-dimensional fluorescence microscopy revealed that ring MCs did not participate in meiotic telomere clustering while MC homologues paired at the XY-body periphery. Self-synapsis of MC(s) and association with the XY-body likely allowed them to pass putative pachytene checkpoints. At metaphase I and II, MC kinetochores assembled MAD2 and BUBR1 spindle checkpoint proteins. Unaligned MCs triggered the spindle checkpoint leading to apoptosis of metaphase cells. Other MCs frequently associated with mouse pericentric heterochromatin, which may have allowed them to pass the spindle checkpoint. Our findings indicate a telomere-independent mechanism for pairing of mammalian MCs, illuminate escape routes to meiotic checkpoints, and give clues for genetic engineering of germ line–permissive chromosomal vectors.

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Language(s): eng - English
 Dates: 2003-09-01
 Publication Status: Issued
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Identifiers: eDoc: 173642
ISI: 000185145100007
DOI: 10.1083/jcb.200305065
 Degree: -

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Title: Journal of Cell Biology
  Alternative Title : J. Cell Biol.
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: -
Pages: - Volume / Issue: 162 (5) Sequence Number: - Start / End Page: 795 - 807 Identifier: ISSN: 0021-9525