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  An enhancer directs differential expression of the linked Mrf4 and Myf5 myogenic regulatory genes in the mouse

Chang, T.-H.-T., Primig, M., Hadchouel, J., Tajbakhsh, S., Rocancourt, D., Fernandez, A., et al. (2004). An enhancer directs differential expression of the linked Mrf4 and Myf5 myogenic regulatory genes in the mouse. Developmental Biology, 269(2), 595-608. doi:10.1016/j.ydbio.2004.02.013.

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Genre: Journal Article
Alternative Title : Dev. Biol.

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 Creators:
Chang, Ted Hung-Tse, Author
Primig, Michael, Author
Hadchouel, Juliette, Author
Tajbakhsh, Shahragim, Author
Rocancourt, Didier, Author
Fernandez, Anne, Author
Kappler, Roland, Author
Scherthan, Harry1, Author           
Buckingham, Margaret, Author
Affiliations:
1Dept. of Human Molecular Genetics (Head: Hans-Hilger Ropers), Max Planck Institute for Molecular Genetics, Max Planck Society, ou_1433549              

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Free keywords: Enhancer; Mrf4K; Myf5; myogenesis; Myogenic regulatory factors
 Abstract: The myogenic regulatory factors, Mrf4 and Myf5, play a key role in skeletal muscle formation. An enhancer trap approach, devised to isolate positive-acting elements from a 200-kb YAC covering the mouse Mrf4–Myf5 locus in a C2 myoblast assay, yielded an enhancer, A17, which mapped at −8 kb 5′ of Mrf4 and −17 kb 5′ of Myf5. An E-box bound by complexes containing the USF transcription factor is critical for enhancer activity. In transgenic mice, A17 gave two distinct and mutually exclusive expression profiles before birth, which correspond to two phases of Mrf4 transcription. Linked to the Tk or Mrf4 minimal promoters, the nlacZ reporter was expressed either in embryonic myotomes, or later in fetal muscle, with the majority of Mrf4 lines showing embryonic expression. When linked to the Myf5 minimal promoter, only fetal muscle expression was detected. These observations identify A17 as a sequence that targets sites of myogenesis in vivo and raise questions about the mutually exclusive modes of expression and possible promoter/enhancer interactions at the Mrf4–Myf5 locus.

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Language(s): eng - English
 Dates: 2004-03-27
 Publication Status: Issued
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Identifiers: eDoc: 224295
DOI: 10.1016/j.ydbio.2004.02.013
 Degree: -

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Title: Developmental Biology
  Alternative Title : Dev. Biol.
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: -
Pages: - Volume / Issue: 269 (2) Sequence Number: - Start / End Page: 595 - 608 Identifier: ISSN: 0012-1606