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  Yersinia enterocolitica type III secretion depends on the proton motive force but not on the flagellar motor components MotA and MotB

Wilharm, G., Lehmann, V., Krauss, K., Lehnert, B., Richter, S., Ruckdeschel, K., et al. (2004). Yersinia enterocolitica type III secretion depends on the proton motive force but not on the flagellar motor components MotA and MotB. Infection and Immunity, 72(7), 4004-4009. doi:10.1128/IAI.72.7.4004-4009.2004.

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Item Permalink: https://hdl.handle.net/11858/00-001M-0000-0010-882A-4 Version Permalink: https://hdl.handle.net/11858/00-001M-0000-0010-882B-2
Genre: Journal Article
Alternative Title : Infect Immun

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 Creators:
Wilharm, Gottfried, Author
Lehmann, Verena1, Author
Krauss, Kristina, Author
Lehnert, Beatrix, Author
Richter, Susanna, Author
Ruckdeschel, Klaus, Author
Heesemann, Jürgen, Author
Trülzsch, Konrad, Author
Affiliations:
1Max Planck Society, ou_persistent13              

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 Abstract: The flagellum is believed to be the common ancestor of all type III secretion systems (TTSSs). In Yersinia enterocolitica, expression of the flagellar TTSS and the Ysc (Yop secretion) TTSS are inversely regulated. We therefore hypothesized that the Ysc TTSS may adopt flagellar motor components in order to use the pathogenicity-related translocon in a drill-like manner. As a prerequisite for this hypothesis, we first tested a requirement for the proton motive force by both systems using the protonophore carbonyl cyanide m-chlorophenylhydrazone (CCCP). Motility as well as type III-dependent secretion of Yop proteins was inhibited by CCCP. We deleted motAB, which resulted in an immotile phenotype. This mutant, however, secreted amounts of Yops to the supernatant comparable to those of the wild type. Translocation of Yops into host cells was also not affected by the motAB deletion. Virulence of the mutant was comparable to that of the wild type in the mouse oral infection model. Thus, the hypothesis that the Ysc TTSS might adopt flagellar motor components was not confirmed. The finding that, in addition to consumption of ATP, Ysc TTSS requires the proton motive force is discussed.

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Language(s): eng - English
 Dates: 2004-07
 Publication Status: Issued
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Identifiers: eDoc: 230844
DOI: 10.1128/IAI.72.7.4004-4009.2004
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Title: Infection and Immunity
  Alternative Title : Infect Immun
Source Genre: Journal
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Pages: - Volume / Issue: 72 (7) Sequence Number: - Start / End Page: 4004 - 4009 Identifier: ISSN: 1098-5522