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キーワード:
affinity enrichment; peptide arrays; phage display; protein design; SPOT synthesis; two dimensional screening
要旨:
Chemically synthesized peptide arrays on planar cellulosecarriers are proposed as libraries of ligands suitable for the multiplexedsimultaneous capture of peptide-specific acceptor proteins from a largerandomly mutagenized library of acceptor proteins presented onbacteriophage M13 particles. This experimental set-up can be exploited to rapidlyscreen for individual new, distinct binding partners from two complementarylibraries (two-dimensional screening). The technical feasibility of thisempirical protein design approach was demonstrated with calmodulin as anaceptor protein using an array of mastoparan variants for multiplexedphage affinity enrichment.
