Deutsch
 
Hilfe Datenschutzhinweis Impressum
  DetailsucheBrowse

Datensatz

 
 
DownloadE-Mail
  Nodal/activin signaling establishes oral-aboral polarity in the early sea urchin embryo

Flowers, V. L., Courteau, G. R., Poustka, A. J., Weng, W., & Venuti, J. M. (2004). Nodal/activin signaling establishes oral-aboral polarity in the early sea urchin embryo. Developmental Dynamics, 231(4), 727-740. doi:10.1002/dvdy.20194.

Item is

Basisdaten

einblenden: ausblenden:
Genre: Zeitschriftenartikel
Alternativer Titel : Dev Dyn

Externe Referenzen

einblenden:

Urheber

einblenden:
ausblenden:
 Urheber:
Flowers, Vera Lynn, Autor
Courteau, Girard R., Autor
Poustka, Albert J.1, Autor           
Weng, Wei, Autor
Venuti, Judith M., Autor
Affiliations:
1Evolution and Development (Albert Poustka), Dept. of Vertebrate Genomics (Head: Hans Lehrach), Max Planck Institute for Molecular Genetics, Max Planck Society, ou_1479650              

Inhalt

einblenden:
ausblenden:
Schlagwörter: -
 Zusammenfassung: Components of the Wnt signaling pathway are involved in patterning the sea urchin primary or animal-vegetal (AV) axis, but the molecular cues that pattern the secondary embryonic axis, the aboral/oral (AO) axis, are not known. In an analysis of signaling molecules that influence patterning along the sea urchin embryonic axes, we found that members of the activin subfamily of transforming growth factor- (TGF-) signaling molecules influence the establishment of AO polarities in the early embryo. Injection of activin mRNAs into fertilized eggs or treatment with exogenously applied recombinant activin altered the allocation of ectodermal fates and ventralized the embryo. The phenotypes observed resemble the ventralized phenotype previously reported for NiCl2, a known disrupter of AO patterning. Sensitivity to exogenous activin occurs between fertilization and the late blastula stage, which is also the time of highest NiCl2 sensitivity. These results argue that specification of fates along the embryonic AO axis involves TGF- signaling. To further examine TGF- signaling in these embryos, we cloned an endogenous TGF- from sea urchin embryos that is a member of the activin subfamily, SpNodal, and show through gain of function analysis that it recapitulates results obtained with exogenous activins and NiCl2. The expression pattern of SpNodal is consistent with a role for nodal signaling in the establishment of fates along the AO axis. Loss of function experiments using SpNodal antisense morpholinos also support a role for SpNodal in the establishment of the AO axis.

Details

einblenden:
ausblenden:
Sprache(n): eng - English
 Datum: 2004-12
 Publikationsstatus: Erschienen
 Seiten: -
 Ort, Verlag, Ausgabe: -
 Inhaltsverzeichnis: -
 Art der Begutachtung: -
 Identifikatoren: eDoc: 230193
DOI: 10.1002/dvdy.20194
 Art des Abschluß: -

Veranstaltung

einblenden:

Entscheidung

einblenden:

Projektinformation

einblenden:

Quelle 1

einblenden:
ausblenden:
Titel: Developmental Dynamics
  Alternativer Titel : Dev Dyn
Genre der Quelle: Zeitschrift
 Urheber:
Affiliations:
Ort, Verlag, Ausgabe: -
Seiten: - Band / Heft: 231 (4) Artikelnummer: - Start- / Endseite: 727 - 740 Identifikator: ISSN: 1058-8388