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Free keywords:
Gene expression; Cell arrays; Transfection; Microarray
Abstract:
RNA interference (RNAi) describes the post-transcriptional silencing of gene expression that occurs in response to the introduction of double-stranded RNA into cells. Application of RNAi in experimental systems has provided a great leap forward in the elucidation of gene function. To facilitate large-scale functional genomics studies using RNAi, several high throughput approaches have been developed based on microarray or microwell assays. Recent establishment of large libraries of RNAi reagents combined with a variety of detection assays further opens the door for genome-wide screens of gene function in mammalian cells.
The review provides a comprehensive overview on current RNA interference-based high throughput genomic technologies. The cell array- and microwell plate-based loss-of-function cell assays are presented.
