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  Genomewide production of multipurpose alleles for the functional analysis of the mouse genome

Schnütgen, F., De-Zolt, S., Van Sloun, P., Hollatz, M., Floss, T., Hansen, J., et al. (2005). Genomewide production of multipurpose alleles for the functional analysis of the mouse genome. Proceedings of the National Academy of Sciences of the United States of America (Washington, DC), 102(20), 7221-7226. doi:10.1073/pnas.0502273102.

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Item Permalink: https://hdl.handle.net/11858/00-001M-0000-0010-865B-6 Version Permalink: https://hdl.handle.net/11858/00-001M-0000-0010-865C-4
Genre: Journal Article
Alternative Title : Proc Natl Acad Sci U S A

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Schnütgen et al. - PNAS.pdf (Any fulltext), 596KB
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 Creators:
Schnütgen, Frank, Author
De-Zolt, Silke, Author
Van Sloun, Petra, Author
Hollatz, Melanie, Author
Floss, Thomas, Author
Hansen, Jens, Author
Altschmied, Joachim, Author
Seisenberger, Claudia, Author
Ghyselinck, Norbert B., Author
Ruiz, Patricia1, Author
Chambon, Pierre, Author
Wurst, Wolfgang, Author
von Melchner, Harald, Author
Affiliations:
1Max Planck Society, ou_persistent13              

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Free keywords: conditional mutagenesis; ES cells; gene trapping; site-specific recombination; insertional mutagenesis
 Abstract: A type of retroviral gene trap vectors has been developed that can induce conditional mutations in most genes expressed in mouse embryonic stem (ES) cells. The vectors rely on directional site-specific recombination systems that can repair and reinduce gene trap mutations when activated in succession. After the gene traps are inserted into the mouse genome, genetic mutations can be produced at a particular time and place in somatic cells. In addition to their conditional features, the vectors create multipurpose alleles amenable to a wide range of postinsertional modifications. Here we have used these directional recombination vectors to assemble the largest library of ES cell lines with conditional mutations in single genes yet assembled, presently totaling 1,000 unique genes. The trapped ES cell lines, which can be ordered from the German Gene Trap Consortium, are freely available to the scientific community.

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Language(s): eng - English
 Dates: 2005-05-03
 Publication Status: Issued
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Identifiers: eDoc: 273184
DOI: 10.1073/pnas.0502273102
 Degree: -

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Title: Proceedings of the National Academy of Sciences of the United States of America (Washington, DC)
  Alternative Title : Proc Natl Acad Sci U S A
Source Genre: Journal
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Pages: - Volume / Issue: 102 (20) Sequence Number: - Start / End Page: 7221 - 7226 Identifier: ISSN: 0027-8424