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  Intronic CA-repeat and CA-rich elements: a new class of regulators of mammalian alternative splicing

Hui, J., Hung, L.-H., Heiner, M., Schreiner, S., Neumüller, N., Reither, G., et al. (2005). Intronic CA-repeat and CA-rich elements: a new class of regulators of mammalian alternative splicing. EMBO Journal, 24(11), 1988-1998. doi:10.1038/sj.emboj.7600677.

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Genre: Journal Article
Alternative Title : EMBO

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 Creators:
Hui, Jingyi, Author
Hung, Lee-Hsueh, Author
Heiner, Monika, Author
Schreiner, Silke, Author
Neumüller, Norma, Author
Reither, Gregor, Author
Haas, Stefan A1, Author
Bindereif, Albrecht, Author
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1Max Planck Society, ou_persistent13              

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Free keywords: alternative splicing hnRNP L repetitive sequence splicing enhancer splicing silencer
 Abstract: We have recently identified an intronic polymorphic CA-repeat region in the human endothelial nitric oxide synthase (eNOS) gene as an important determinant of the splicing efficiency, requiring specific binding of hnRNP L. Here, we analyzed the position requirements of this CA-repeat element, which revealed its potential role in alternative splicing. In addition, we defined the RNA binding specificity of hnRNP L by SELEX: not only regular CA repeats are recognized with high affinity but also certain CA-rich clusters. Therefore, we have systematically searched the human genome databases for CA-repeat and CA-rich elements associated with alternative 5' splice sites (5'ss), followed by minigene transfection assays. Surprisingly, in several specific human genes that we tested, intronic CA RNA elements could function either as splicing enhancers or silencers, depending on their proximity to the alternative 5'ss. HnRNP L was detected specifically bound to these diverse CA elements. These data demonstrated that intronic CA sequences constitute novel and widespread regulatory elements of alternative splicing.

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Language(s): eng - English
 Dates: 2005-05-12
 Publication Status: Issued
 Pages: -
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 Table of Contents: -
 Rev. Type: -
 Identifiers: eDoc: 265100
DOI: 10.1038/sj.emboj.7600677
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Title: EMBO Journal
  Alternative Title : EMBO
Source Genre: Journal
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Pages: - Volume / Issue: 24 (11) Sequence Number: - Start / End Page: 1988 - 1998 Identifier: ISSN: 0261 - 4189