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  Structural genomics of human proteins – target selection and generation of a public catalogue of expression clones

Büssow, K., Scheich, C., Sievert, V., Harttig, U., Schultz, J., Simon, B., et al. (2005). Structural genomics of human proteins – target selection and generation of a public catalogue of expression clones. Microbial Cell Factories, Microb Cell Fact(4:21), 1-13. doi:10.1186/1475-2859-4-21.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0010-8606-2 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0010-8607-F
Genre: Journal Article

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Büssow et al. - Microb Cell Fact.pdf (Any fulltext), 663KB
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 Creators:
Büssow, Konrad1, Author              
Scheich, Christoph2, Author
Sievert, Volker1, Author              
Harttig, Ulrich, Author
Schultz, Jörg, Author
Simon, Bernd, Author
Bork, Peer, Author
Lehrach, Hans1, Author              
Heinemann, Udo, Author
Affiliations:
1Dept. of Vertebrate Genomics (Head: Hans Lehrach), Max Planck Institute for Molecular Genetics, Max Planck Society, escidoc:1433550              
2Max Planck Society, escidoc:persistent13              

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 Abstract: Background The availability of suitable recombinant protein is still a major bottleneck in protein structure analysis. The Protein Structure Factory, part of the international structural genomics initiative, targets human proteins for structure determination. It has implemented high throughput procedures for all steps from cloning to structure calculation. This article describes the selection of human target proteins for structure analysis, our high throughput cloning strategy, and the expression of human proteins in Escherichia coli host cells. Results and Conclusion Protein expression and sequence data of 1414 E. coli expression clones representing 537 different proteins are presented. 139 human proteins (18%) could be expressed and purified in soluble form and with the expected size. All E. coli expression clones are publicly available to facilitate further functional characterisation of this set of human proteins.

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Language(s): eng - English
 Dates: 2005-07-05
 Publication Status: Published in print
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 Identifiers: eDoc: 268551
DOI: 10.1186/1475-2859-4-21
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Title: Microbial Cell Factories
Source Genre: Journal
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Pages: - Volume / Issue: Microb Cell Fact (4:21) Sequence Number: - Start / End Page: 1 - 13 Identifier: ISSN: 1475-2859