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  High-throughput trapping of secretory pathway genes in mouse embryonic stem cells

De-Zolt, S., Schnütgen, F., Seisenberger, C., Hansen, J., Hollatz, M., Floss, T., et al. (2006). High-throughput trapping of secretory pathway genes in mouse embryonic stem cells. Nucleic Acids Research (London), 34(3), e25-e25. doi:10.1093/nar/gnj026.

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Item Permalink: https://hdl.handle.net/11858/00-001M-0000-0010-8492-7 Version Permalink: https://hdl.handle.net/11858/00-001M-0000-0010-8493-5
Genre: Journal Article
Alternative Title : Nucleic Acids Res

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 Creators:
De-Zolt, Silke, Author
Schnütgen, Frank, Author
Seisenberger, Claudia, Author
Hansen, Jens, Author
Hollatz, Melanie, Author
Floss, Thomas, Author
Ruiz, Patricia1, Author
Wurst, Wolfgang, Author
von Melchner, Harald, Author
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1Max Planck Society, ou_persistent13              

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 Abstract: High-throughput gene trapping is a random approach for inducing insertional mutations across the mouse genome. This approach uses gene trap vectors that simultaneously inactivate and report the expression of the trapped gene at the insertion site, and provide a DNA tag for the rapid identification of the disrupted gene. Gene trapping has been used by both public and private institutions to produce libraries of embryonic stem (ES) cells harboring mutations in single genes. Presently, ~66% of the protein coding genes in the mouse genome have been disrupted by gene trap insertions. Among these, however, genes encoding signal peptides or transmembrane domains (secretory genes) are underrepresented because they are not susceptible to conventional trapping methods. Here, we describe a high-throughput gene trapping strategy that effectively targets secretory genes. We used this strategy to assemble a library of ES cells harboring mutations in 716 unique secretory genes, of which 61% were not trapped by conventional trapping, indicating that the two strategies are complementary. The trapped ES cell lines, which can be ordered from the International Gene Trap Consortium (http://www.genetrap.org), are freely available to the scientific community.

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Language(s): eng - English
 Dates: 2006-02-13
 Publication Status: Issued
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 Rev. Type: -
 Identifiers: eDoc: 307432
DOI: 10.1093/nar/gnj026
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Title: Nucleic Acids Research (London)
  Alternative Title : Nucleic Acids Res
Source Genre: Journal
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Pages: - Volume / Issue: 34 (3) Sequence Number: - Start / End Page: e25 - e25 Identifier: ISSN: 0305-1048