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  Nonribosomal peptide synthesis in S. pombe and the architecture of ferrichrome-type siderophore synthetases in fungi

Schwecke, T., Göttling, K., Durek, P., Dueñas, I., Käufer, N. F., Zock-Emmenthal, S., et al. (2006). Nonribosomal peptide synthesis in S. pombe and the architecture of ferrichrome-type siderophore synthetases in fungi. ChemBioChem: A European Journal of Chemical Biology, 7(4), 612-622. doi:10.1002/cbic.200500301.

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Item Permalink: https://hdl.handle.net/11858/00-001M-0000-0010-8486-3 Version Permalink: https://hdl.handle.net/11858/00-001M-0000-0010-8487-1
Genre: Journal Article
Alternative Title : ChemBioChem

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fulltext_ID=112467109&PLACEBO=IE.pdf (Any fulltext), 651KB
 
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 Creators:
Schwecke,, Torsten, Author
Göttling, Kirsten, Author
Durek, Pawel, Author
Dueñas, Ines, Author
Käufer, Norbert F., Author
Zock-Emmenthal, Susanne, Author
Staub, Eike1, Author
Neuhof, Torsten, Author
Dieckmann, Ralf1, Author
von Döhren, Hans, Author
Affiliations:
1Max Planck Society, ou_persistent13              

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Free keywords: biosynthesis • evolution • ferrichrome • fungi • nonribosomal peptide synthetases
 Abstract: A nonribosomal peptide synthetase (NRPS) in Schizosaccharomyces pombe, which possesses an unusual structure incorporating three adenylation domains, six thiolation domains and six condensation domains, has been shown to produce the cyclohexapeptide siderophore ferrichrome. One of the adenylation domains is truncated and contains a distorted key motif. Substrate-binding specificities of the remaining two domains were assigned by molecular modelling to glycine and to N-acetyl-N-hydroxy-L-ornithine. Hexapeptide siderophore synthetase genes of Magnaporthe grisea and Fusarium graminearum were both identified and analyzed with respect to substrate-binding sites, and the predicted product ferricrocin was identified in each. A comparative analysis of these synthetase systems, including those of the basidiomycete Ustilago maydis, the homobasidiomycete Omphalotus olearius and the ascomycetes Aspergillus nidulans, Aspergillus fumigatus, Fusarium graminearum, Cochliobolus heterostrophus, Neurospora crassa and Aureobasidium pullulans, revealed divergent domain compositions with respect to their number and positioning, although all produce similar products by iterative processes. A phylogenetic analysis of both NRPSs and associated L-N5-ornithine monooxygenases revealed that ferrichrome-type siderophore biosynthesis has coevolved in fungi with varying in trans interactions of NRPS domains.

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Language(s): eng - English
 Dates: 2006-02-27
 Publication Status: Issued
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 Identifiers: eDoc: 311430
DOI: 10.1002/cbic.200500301
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Title: ChemBioChem : A European Journal of Chemical Biology
  Alternative Title : ChemBioChem
Source Genre: Journal
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Pages: - Volume / Issue: 7 (4) Sequence Number: - Start / End Page: 612 - 622 Identifier: ISSN: 1439-7633