Transcriptional census of 36 microdissected colorectal cancers yields a gene 
signature to distinguish UICC II and III

Groene, Joern; Mansmann, Ulrich; Meister, Reinhard; Staub, Eike; Roepcke, Stefan; Heinze, Maya; Klaman, Irina; Brümmendorf, Thomas; Hermann, Klaus; Loddenkemper, Christoph; Pilarsky, Christian; Mann, Benno; Adams, Hans-Peter; Buhr, Heinz Johannes; Rosenthal, André

doi:10.1002/ijc.22027

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Transcriptional census of 36 microdissected colorectal cancers yields a gene signature to distinguish UICC II and III

Groene, J., Mansmann, U., Meister, R., Staub, E., Roepcke, S., Heinze, M., et al. (2006). Transcriptional census of 36 microdissected colorectal cancers yields a gene signature to distinguish UICC II and III. International Journal of Cancer, 119(8), 1829-1836. doi:10.1002/ijc.22027.

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Item Permalink: https://hdl.handle.net/11858/00-001M-0000-0010-8428-6 Version Permalink: https://hdl.handle.net/11858/00-001M-0000-0010-8429-4

Genre: Journal Article

Alternative Title : Int. J. Cancer

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Creators:
Groene, Joern, Author
Mansmann, Ulrich, Author
Meister, Reinhard, Author
Staub, Eike¹, Author
Roepcke, Stefan¹, Author
Heinze, Maya, Author
Klaman, Irina, Author
Brümmendorf, Thomas, Author
Hermann, Klaus, Author
Loddenkemper, Christoph, Author
Pilarsky, Christian, Author
Mann, Benno, Author
Adams, Hans-Peter, Author
Buhr, Heinz Johannes, Author
Rosenthal, André, Author

Affiliations:
1Max Planck Society, ou_persistent13

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Free keywords: colorectal cancer • gene expression analysis • gene signature • classification • UICC stage II • UICC stage III

Abstract: UICC stage II and III colorectal cancers (CRC) differ fundamentally in prognosis and therapeutic concepts. To analyze differential gene expression between both stages and to establish a relationship between molecular background and clinical presentation, tumor material from 36 unselected consecutive patients presenting with sporadic CRC, 18 UICC stage II and 18 UICC stage III, were laser microdissected to separate epithelial tumor cells. Gene expression levels were measured using U133A Affymetrix gene arrays. Twelve CRC associated signal transduction pathways as well as all 22,000 probe sets were screened for differential gene expression. We identified a signature consisting of 45 probe sets that allowed discrimination between UICC stage II and stage III with a rate of correct classification of about 80%. The most distinctive elements in this signature were the gene GSTP-binding elongation factor (GSPT2) and the transcription factor HOXA9. Differential expression of these genes was confirmed by quantitative real-time polymerase chain reaction (p(HOXA9) = 0.04, p(GSTP2) = 0.02). Despite the reliability of the presented data, there was no substantial differential expression of genes in cancer-related pathways. However, the comparison with recently published data corroborates the 45 gene signature showing structural agreement in the direction of fold changes of gene expression levels for our set of genes chosen to discriminate between both stages

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Language(s): eng - English

Dates: Date issued: 2006-05-23

Publication Status: Issued

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Identifiers: eDoc: 309274
DOI: 10.1002/ijc.22027

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Title: International Journal of Cancer

Alternative Title : Int. J. Cancer

Source Genre: Journal

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Pages: - Volume / Issue: 119 (8) Sequence Number: - Start / End Page: 1829 - 1836 Identifier: ISSN: 0020-7136