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  Transcriptional census of 36 microdissected colorectal cancers yields a gene signature to distinguish UICC II and III

Groene, J., Mansmann, U., Meister, R., Staub, E., Roepcke, S., Heinze, M., et al. (2006). Transcriptional census of 36 microdissected colorectal cancers yields a gene signature to distinguish UICC II and III. International Journal of Cancer, 119(8), 1829-1836. doi:10.1002/ijc.22027.

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Genre: Zeitschriftenartikel
Alternativer Titel : Int. J. Cancer

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 Urheber:
Groene, Joern, Autor
Mansmann, Ulrich, Autor
Meister, Reinhard, Autor
Staub, Eike1, Autor
Roepcke, Stefan1, Autor
Heinze, Maya, Autor
Klaman, Irina, Autor
Brümmendorf, Thomas, Autor
Hermann, Klaus, Autor
Loddenkemper, Christoph, Autor
Pilarsky, Christian, Autor
Mann, Benno, Autor
Adams, Hans-Peter, Autor
Buhr, Heinz Johannes, Autor
Rosenthal, André, Autor
Affiliations:
1Max Planck Society, ou_persistent13              

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Schlagwörter: colorectal cancer • gene expression analysis • gene signature • classification • UICC stage II • UICC stage III
 Zusammenfassung: UICC stage II and III colorectal cancers (CRC) differ fundamentally in prognosis and therapeutic concepts. To analyze differential gene expression between both stages and to establish a relationship between molecular background and clinical presentation, tumor material from 36 unselected consecutive patients presenting with sporadic CRC, 18 UICC stage II and 18 UICC stage III, were laser microdissected to separate epithelial tumor cells. Gene expression levels were measured using U133A Affymetrix gene arrays. Twelve CRC associated signal transduction pathways as well as all 22,000 probe sets were screened for differential gene expression. We identified a signature consisting of 45 probe sets that allowed discrimination between UICC stage II and stage III with a rate of correct classification of about 80%. The most distinctive elements in this signature were the gene GSTP-binding elongation factor (GSPT2) and the transcription factor HOXA9. Differential expression of these genes was confirmed by quantitative real-time polymerase chain reaction (p(HOXA9) = 0.04, p(GSTP2) = 0.02). Despite the reliability of the presented data, there was no substantial differential expression of genes in cancer-related pathways. However, the comparison with recently published data corroborates the 45 gene signature showing structural agreement in the direction of fold changes of gene expression levels for our set of genes chosen to discriminate between both stages

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Sprache(n): eng - English
 Datum: 2006-05-23
 Publikationsstatus: Erschienen
 Seiten: -
 Ort, Verlag, Ausgabe: -
 Inhaltsverzeichnis: -
 Art der Begutachtung: -
 Identifikatoren: eDoc: 309274
DOI: 10.1002/ijc.22027
 Art des Abschluß: -

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Titel: International Journal of Cancer
  Alternativer Titel : Int. J. Cancer
Genre der Quelle: Zeitschrift
 Urheber:
Affiliations:
Ort, Verlag, Ausgabe: -
Seiten: - Band / Heft: 119 (8) Artikelnummer: - Start- / Endseite: 1829 - 1836 Identifikator: ISSN: 0020-7136