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  Following the signal sequence from ribosomal tunnel exit to signal recognition particle

Halic, M., Blau, M., Becker, T., Mielke, T., Pool, M. R., Wild, K., et al. (2006). Following the signal sequence from ribosomal tunnel exit to signal recognition particle. Nature, 444(7118), 507-511. doi:10.1038/nature05326.

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Halic, Mario, Author
Blau, Michael, Author
Becker, Thomas, Author
Mielke, Thorsten1, Author           
Pool, Martin R., Author
Wild, Klemens, Author
Sinning, Irmgard, Author
Beckmann, Roland2, Author
Affiliations:
1Imaging/Electron Microscopy (Head: Rudi Lurz/Thorsten Mielke), Scientific Service (Head: Manuela B. Urban), Max Planck Institute for Molecular Genetics, Max Planck Society, ou_1479668              
2Max Planck Society, ou_persistent13              

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 Abstract: Membrane and secretory proteins can be co-translationally inserted into or translocated across the membrane. This process is dependent on signal sequence recognition on the ribosome by the signal recognition particle (SRP), which results in targeting of the ribosome–nascent-chain complex to the protein-conducting channel at the membrane. Here we present an ensemble of structures at subnanometre resolution, revealing the signal sequence both at the ribosomal tunnel exit and in the bacterial and eukaryotic ribosome–SRP complexes. Molecular details of signal sequence interaction in both prokaryotic and eukaryotic complexes were obtained by fitting high-resolution molecular models. The signal sequence is presented at the ribosomal tunnel exit in an exposed position ready for accommodation in the hydrophobic groove of the rearranged SRP54 M domain. Upon ribosome binding, the SRP54 NG domain also undergoes a conformational rearrangement, priming it for the subsequent docking reaction with the NG domain of the SRP receptor. These findings provide the structural basis for improving our understanding of the early steps of co-translational protein sorting.

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Language(s): eng - English
 Dates: 2006-11-23
 Publication Status: Issued
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 Identifiers: eDoc: 307700
DOI: 10.1038/nature05326
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Title: Nature
Source Genre: Journal
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Pages: - Volume / Issue: 444 (7118) Sequence Number: - Start / End Page: 507 - 511 Identifier: ISSN: 0028-0836