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  Ligand bound beta1 integrins inhibit procaspase-8 for mediating cell adhesion-mediated drug and radiation resistance in human leukemia cells

Estrugo, D., Fischer, A., Hess, F., Scherthan, H., Belka, C., & Nils Cordes, N. (2007). Ligand bound beta1 integrins inhibit procaspase-8 for mediating cell adhesion-mediated drug and radiation resistance in human leukemia cells. PLoS One, 2(3), e269-e269. Retrieved from 10.1371/journal.pone.0000269.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0010-8224-B Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0010-8225-9
Genre: Journal Article

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picrender.pdf (Any fulltext), 876KB
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Estrugo, Doris, Author
Fischer, Alexander, Author
Hess, Franziska, Author
Scherthan, Harry1, Author              
Belka, Claus, Author
Nils Cordes, Nils, Author
Affiliations:
1Dept. of Human Molecular Genetics (Head: Hans-Hilger Ropers), Max Planck Institute for Molecular Genetics, Max Planck Society, escidoc:1433549              

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 Abstract: Background Chemo- and radiotherapeutic responses of leukemia cells are modified by integrin-mediated adhesion to extracellular matrix. To further characterize the molecular mechanisms by which β1 integrins confer radiation and chemoresistance, HL60 human acute promyelocytic leukemia cells stably transfected with β1 integrin and A3 Jurkat T-lymphoma cells deficient for Fas-associated death domain protein or procaspase-8 were examined. Methodology/Principal Findings Upon exposure to X-rays, Ara-C or FasL, suspension and adhesion (fibronectin (FN), laminin, collagen-1; 5–100 µg/cm2 coating concentration) cultures were processed for measurement of apoptosis, mitochondrial transmembrane potential (MTP), caspase activation, and protein analysis. Overexpression of β1 integrins enhanced the cellular sensitivity to X-rays and Ara-C, which was counteracted by increasing concentrations of matrix proteins in association with reduced caspase-3 and -8 activation and MTP breakdown. Usage of stimulatory or inhibitory anti β1 integrin antibodies, pharmacological caspase or phosphatidylinositol-3 kinase (PI3K) inhibitors, coprecipitation experiments and siRNA-mediated β1 integrin silencing provided further data showing an interaction between FN-ligated β1 integrin and PI3K/Akt for inhibiting procaspase-8 cleavage. Conclusions/Significance The presented data suggest that the ligand status of β1 integrins is critical for their antiapoptotic effect in leukemia cells treated with Ara-C, FasL or ionizing radiation. The antiapoptotic actions involve formation of a β1 integrin/Akt complex, which signals to prevent procaspase-8-mediated induction of apoptosis in a PI3K-dependent manner. Antagonizing agents targeting β1 integrin and PI3K/Akt signaling in conjunction with conventional therapies might effectively reduce radiation- and drug-resistant tumor populations and treatment failure in hematological malignancies.

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Language(s): eng - English
 Dates: 2007-03-07
 Publication Status: Published in print
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 Identifiers: eDoc: 334027
URI: 10.1371/journal.pone.0000269.
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Title: PLoS One
Source Genre: Journal
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Pages: - Volume / Issue: 2 (3) Sequence Number: - Start / End Page: e269 - e269 Identifier: ISSN: 1932-6203