English
 
Help Privacy Policy Disclaimer
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  Functional proteomic view of metabolic regulation in "Aromatoleum aromaticum" strain EbN1.

Wöhlbrand, L., Kallerhoff, B., Lange, D., Hufnagel, P., Thiermann, J., Reinhardt, R., et al. (2007). Functional proteomic view of metabolic regulation in "Aromatoleum aromaticum" strain EbN1. PROTEOMICS, 7(13), 2222-2239. doi:10.1002/pmic.200600987.

Item is

Basic

show hide
Genre: Journal Article
Alternative Title : PROTEOMICS

Files

show Files
hide Files
:
fulltext_ID=114276796&PLACEBO=IE.pdf (Any fulltext), 2MB
 
File Permalink:
-
Name:
fulltext_ID=114276796&PLACEBO=IE.pdf
Description:
-
OA-Status:
Visibility:
Restricted (Max Planck Institute for Molecular Genetics, MBMG; )
MIME-Type / Checksum:
application/pdf
Technical Metadata:
Copyright Date:
-
Copyright Info:
eDoc_access: MPG
License:
-

Locators

show

Creators

show
hide
 Creators:
Wöhlbrand, Lars1, Author
Kallerhoff, Birte1, Author
Lange, Daniela1, Author
Hufnagel, Peter, Author
Thiermann, Jürgen, Author
Reinhardt, Richard2, Author           
Rabus, Ralf1, Author
Affiliations:
1Max Planck Society, ou_persistent13              
2High Throughput Technologies, Max Planck Institute for Molecular Genetics, Max Planck Society, ou_1433552              

Content

show
hide
Free keywords: Anaerobic biodegradation • Aromatic compounds • Fluorescence two-dimensional difference gel electrophoresis • Functional proteomics • Mass spectrometry
 Abstract: The denitrifying Aromatoleum aromaticum strain EbN1 utilizes a wide range of aromatic and nonaromatic compounds under anoxic and oxic conditions. The recently determined genome revealed corresponding degradation pathways and predicted a fine-tuned regulatory network. In this study, differential proteomics (2-D DIGE and MS) was used to define degradation pathway-specific subproteomes and to determine their growth condition dependent regulation. Differential protein profiles were determined for cultures adapted to growth under 22 different substrate and redox conditions. In total, 354 different proteins were identified, 199 of which displayed significantly changed abundances. These regulated proteins mainly represented enzymes of the different degradation pathways, and revealed different degrees of growth condition specific regulation. In case of three substrate conditions (e.g. phenylalanine, anoxic), proteins previously predicted to be involved in their degradation were apparently not involved (e.g. Pdh, phenylacetaldehyde dehydrogenase). Instead, previously not considered proteins were specifically increased in abundance (e.g. EbA5005, predicted aldehyde:ferredoxin oxidoreductase), shedding new light on the respective pathways. Moreover, strong evidence was obtained for thus far unpredicted degradation pathways of three hitherto unknown substrates (e.g. o-aminobenzoate, anoxic). Comparing all identified regulated and nonregulated proteins provided first insights into regulatory hierarchies of special degradation pathways versus general metabolism in strain EbN1.

Details

show
hide
Language(s): eng - English
 Dates: 2007-06-05
 Publication Status: Issued
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: -
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: PROTEOMICS
  Alternative Title : PROTEOMICS
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: -
Pages: - Volume / Issue: 7 (13) Sequence Number: - Start / End Page: 2222 - 2239 Identifier: ISSN: 1615-9853