de.mpg.escidoc.pubman.appbase.FacesBean
English
 
Help Guide Disclaimer Contact us Login
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
 
 
 
 
DownloadE-Mail
  Temporal transcriptomic analysis of the Listeria monocytogenes EGD-e sigmaB regulon

Hain, T., Hossain, H., Chatterjee, S. S., Machata, S., Volk, U., Wagner, S., et al. (2008). Temporal transcriptomic analysis of the Listeria monocytogenes EGD-e sigmaB regulon. BMC Microbiology, 8(20). doi:10.1186/1471-2180-8-20.

Item is

Basic

show hide
Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0010-8079-C Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0010-807A-A
Genre: Journal Article

Files

show Files

Locators

show

Creators

show
hide
 Creators:
Hain, Torsten, Author
Hossain, Hamid, Author
Chatterjee, Som S, Author
Machata, Silke, Author
Volk, Ute, Author
Wagner, Sandra, Author
Brors, Benedikt, Author
Haas, Stefan1, Author              
Kuenne, Carsten T, Author
Billion, Andre, Author
Otten, Sonja, Author
Pane-Farre, Jan, Author
Engelmann, Susanne, Author
Chakraborty, Trinad, Author
Affiliations:
1Gene Structure and Array Design (Stefan Haas), Dept. of Computational Molecular Biology (Head: Martin Vingron), Max Planck Institute for Molecular Genetics, Max Planck Society, escidoc:1479640              

Content

show
hide
Free keywords: -
 Abstract: Background The opportunistic food-borne gram-positive pathogen Listeria monocytogenes can exist as a free-living microorganism in the environment and grow in the cytoplasm of vertebrate and invertebrate cells following infection. The general stress response, controlled by the alternative sigma factor, σB, has an important role for bacterial survival both in the environment and during infection. We used quantitative real-time PCR analysis and immuno-blot analysis to examine σB expression during growth of L. monocytogenes EGD-e. Whole genome-based transcriptional profiling was used to identify σB-dependent genes at different growth phases. Results We detected 105 σB-positively regulated genes and 111 genes which appeared to be under negative control of σB and validated 36 σB-positively regulated genes in vivo using a reporter gene fusion system. Conclusion Genes comprising the σB regulon encode solute transporters, novel cell-wall proteins, universal stress proteins, transcriptional regulators and include those involved in osmoregulation, carbon metabolism, ribosome- and envelope-function, as well as virulence and niche-specific survival genes such as those involved in bile resistance and exclusion. Ten of the σB-positively regulated genes of L. monocytogenes are absent in L. innocua. A total of 75 σB-positively regulated listerial genes had homologs in B. subtilis, but only 33 have been previously described as being σB-regulated in B. subtilis even though both species share a highly conserved σB-dependent consensus sequence. A low overlap of genes may reflects adaptation of these bacteria to their respective environmental conditions.

Details

show
hide
Language(s): eng - English
 Dates: 2008-01-28
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Method: -
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: BMC Microbiology
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: -
Pages: - Volume / Issue: 8 (20) Sequence Number: - Start / End Page: - Identifier: -