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Free keywords:
Rolling circle amplification, Fluorescence, Biochips, Microarrays, Signal amplification, Cy3-labeled oligonucleotides, DNA technology
Abstract:
Microarrays have become an increasingly important tool for biotechnology and molecular diagnostics. Despite many advantages, their sensitivity is still insufficient for such tasks as the analysis of small sample quantities and for the detection of alterations in gene expression of low-abundance genes. Accordingly, amplification strategies are necessary. Approaches to amplify the signal intensity include the increase of the number of dye molecules per target through either particle labels or rolling circle amplification, as used for this study.
