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  Toward improved biochips based on rolling circle amplification—influences of the microenvironment on the fluorescence properties of labeled DNA oligonucleotides

Mayer-Enthart, E., Sialelli, J., Rurack, K., Resch-Genger, U., Köster, D., & Seitz, H. (2008). Toward improved biochips based on rolling circle amplification—influences of the microenvironment on the fluorescence properties of labeled DNA oligonucleotides. Annals of the New York Academy of Sciences, 1130(1), 287-292. doi:10.1196/annals.1430.022.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0010-7FFE-9 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0010-7FFF-7
Genre: Journal Article
Alternative Title : Ann N Y Acad Sci

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 Creators:
Mayer-Enthart, Elke, Author
Sialelli, Julien, Author
Rurack, Knut, Author
Resch-Genger, Ute, Author
Köster, Daniela1, Author
Seitz, Harald2, Author              
Affiliations:
1Max Planck Society, escidoc:persistent13              
2Dept. of Vertebrate Genomics (Head: Hans Lehrach), Max Planck Institute for Molecular Genetics, Max Planck Society, escidoc:1433550              

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Free keywords: Rolling circle amplification, Fluorescence, Biochips, Microarrays, Signal amplification, Cy3-labeled oligonucleotides, DNA technology
 Abstract: Microarrays have become an increasingly important tool for biotechnology and molecular diagnostics. Despite many advantages, their sensitivity is still insufficient for such tasks as the analysis of small sample quantities and for the detection of alterations in gene expression of low-abundance genes. Accordingly, amplification strategies are necessary. Approaches to amplify the signal intensity include the increase of the number of dye molecules per target through either particle labels or rolling circle amplification, as used for this study.

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Language(s): eng - English
 Dates: 2008-05
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Method: -
 Degree: -

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Title: Annals of the New York Academy of Sciences
  Alternative Title : Ann N Y Acad Sci
Source Genre: Journal
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Affiliations:
Publ. Info: -
Pages: - Volume / Issue: 1130 (1) Sequence Number: - Start / End Page: 287 - 292 Identifier: ISSN: 1749-6632