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  Streptococcus pyogenes pSM19035 requires dynamic assembly of ATP-bound ParA and ParB on parS DNA during plasmid segregation

Pratto, F., Cicek, A., Weihofen, W. A., Lurz, R., Saenger, W., & Alonso, J. C. (2008). Streptococcus pyogenes pSM19035 requires dynamic assembly of ATP-bound ParA and ParB on parS DNA during plasmid segregation. Nucleic Acids Research, 36(11), 3676-3689. doi:10.1093/nar/gkn170.

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Item Permalink: https://hdl.handle.net/11858/00-001M-0000-0010-7FDC-6 Version Permalink: https://hdl.handle.net/11858/00-001M-0000-0010-7FDD-4
Genre: Journal Article
Alternative Title : Nucleic Acids Res.

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Pratto, Florencia, Author
Cicek, Aslan, Author
Weihofen, Wilhelm A., Author
Lurz, Rudi1, Author
Saenger, Wolfram, Author
Alonso, Juan C., Author
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1Max Planck Society, ou_persistent13              

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 Abstract: The accurate partitioning of Firmicute plasmid pSM19035 at cell division depends on ATP binding and hydrolysis by homodimeric ATPase {delta}2 (ParA) and binding of {omega}2 (ParB) to its cognate parS DNA. The 1.83 Å resolution crystal structure of {delta}2 in a complex with non-hydrolyzable ATP{gamma}S reveals a unique ParA dimer assembly that permits nucleotide exchange without requiring dissociation into monomers. In vitro, {delta}2 had minimal ATPase activity in the absence of {omega}2 and parS DNA. However, stoichiometric amounts of {omega}2 and parS DNA stimulated the {delta}2 ATPase activity and mediated plasmid pairing, whereas at high (4:1) {omega}2 : {delta}2 ratios, stimulation of the ATPase activity was reduced and {delta}2 polymerized onto DNA. Stimulation of the {delta}2 ATPase activity and its polymerization on DNA required ability of {omega}2 to bind parS DNA and its N-terminus. In vivo experiments showed that {delta}2 alone associated with the nucleoid, and in the presence of {omega}2 and parS DNA, {delta}2 oscillated between the nucleoid and the cell poles and formed spiral-like structures. Our studies indicate that the molar {omega}2 : {delta}2 ratio regulates the polymerization properties of ({delta}•ATP•Mg2+)2 on and depolymerization from parS DNA, thereby controlling the temporal and spatial segregation of pSM19035 before cell division.

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Language(s): eng - English
 Dates: 2008-05-13
 Publication Status: Issued
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 Identifiers: eDoc: 412078
URI: http://nar.oxfordjournals.org/cgi/reprint/36/11/3676
DOI: 10.1093/nar/gkn170
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Title: Nucleic Acids Research
  Alternative Title : Nucleic Acids Res.
Source Genre: Journal
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Pages: - Volume / Issue: 36 (11) Sequence Number: - Start / End Page: 3676 - 3689 Identifier: ISSN: 0305-1048