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  Streptococcus pyogenes pSM19035 requires dynamic assembly of ATP-bound ParA and ParB on parS DNA during plasmid segregation

Pratto, F., Cicek, A., Weihofen, W. A., Lurz, R., Saenger, W., & Alonso, J. C. (2008). Streptococcus pyogenes pSM19035 requires dynamic assembly of ATP-bound ParA and ParB on parS DNA during plasmid segregation. Nucleic Acids Research, 36(11), 3676-3689. doi:10.1093/nar/gkn170.

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Datensatz-Permalink: https://hdl.handle.net/11858/00-001M-0000-0010-7FDC-6 Versions-Permalink: https://hdl.handle.net/11858/00-001M-0000-0010-7FDD-4
Genre: Zeitschriftenartikel
Alternativer Titel : Nucleic Acids Res.

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 Urheber:
Pratto, Florencia, Autor
Cicek, Aslan, Autor
Weihofen, Wilhelm A., Autor
Lurz, Rudi1, Autor
Saenger, Wolfram, Autor
Alonso, Juan C., Autor
Affiliations:
1Max Planck Society, ou_persistent13              

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 Zusammenfassung: The accurate partitioning of Firmicute plasmid pSM19035 at cell division depends on ATP binding and hydrolysis by homodimeric ATPase {delta}2 (ParA) and binding of {omega}2 (ParB) to its cognate parS DNA. The 1.83 Å resolution crystal structure of {delta}2 in a complex with non-hydrolyzable ATP{gamma}S reveals a unique ParA dimer assembly that permits nucleotide exchange without requiring dissociation into monomers. In vitro, {delta}2 had minimal ATPase activity in the absence of {omega}2 and parS DNA. However, stoichiometric amounts of {omega}2 and parS DNA stimulated the {delta}2 ATPase activity and mediated plasmid pairing, whereas at high (4:1) {omega}2 : {delta}2 ratios, stimulation of the ATPase activity was reduced and {delta}2 polymerized onto DNA. Stimulation of the {delta}2 ATPase activity and its polymerization on DNA required ability of {omega}2 to bind parS DNA and its N-terminus. In vivo experiments showed that {delta}2 alone associated with the nucleoid, and in the presence of {omega}2 and parS DNA, {delta}2 oscillated between the nucleoid and the cell poles and formed spiral-like structures. Our studies indicate that the molar {omega}2 : {delta}2 ratio regulates the polymerization properties of ({delta}•ATP•Mg2+)2 on and depolymerization from parS DNA, thereby controlling the temporal and spatial segregation of pSM19035 before cell division.

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Sprache(n): eng - English
 Datum: 2008-05-13
 Publikationsstatus: Erschienen
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 Ort, Verlag, Ausgabe: -
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 Identifikatoren: eDoc: 412078
URI: http://nar.oxfordjournals.org/cgi/reprint/36/11/3676
DOI: 10.1093/nar/gkn170
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Titel: Nucleic Acids Research
  Alternativer Titel : Nucleic Acids Res.
Genre der Quelle: Zeitschrift
 Urheber:
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Ort, Verlag, Ausgabe: -
Seiten: - Band / Heft: 36 (11) Artikelnummer: - Start- / Endseite: 3676 - 3689 Identifikator: ISSN: 0305-1048