Deutsch
 
Hilfe Datenschutzhinweis Impressum
  DetailsucheBrowse

Datensatz

 
 
DownloadE-Mail
  New features of the ribosome and ribosomal inhibitors: non-enzymatic recycling, misreading and back-translocation

Szaflarski, W., Vesper, O., Teraoka, Y., Plitta, B., Wilson, D. N., & Nierhaus, K. H. (2008). New features of the ribosome and ribosomal inhibitors: non-enzymatic recycling, misreading and back-translocation. Journal of Molecular Biology, 380(1), 193-205. doi:10.1016/j.jmb.2008.04.060.

Item is

Basisdaten

einblenden: ausblenden:
Genre: Zeitschriftenartikel
Alternativer Titel : J Mol Biol

Externe Referenzen

einblenden:

Urheber

einblenden:
ausblenden:
 Urheber:
Szaflarski, Witold1, Autor           
Vesper, Oliver1, Autor           
Teraoka, Yoshika2, Autor
Plitta, Beata2, Autor
Wilson, Daniel N.3, Autor           
Nierhaus, Knud H.1, Autor           
Affiliations:
1Ribosomes, Max Planck Institute for Molecular Genetics, Max Planck Society, ou_1433558              
2Max Planck Society, ou_persistent13              
3Dept. of Vertebrate Genomics (Head: Hans Lehrach), Max Planck Institute for Molecular Genetics, Max Planck Society, ou_1433550              

Inhalt

einblenden:
ausblenden:
Schlagwörter: aminoglycosides; NRI drug, naphthyridine, inhibition mechanism; optimal poly(Phe) synthesis system; recycling of ribosomes; back-translocation
 Zusammenfassung: We describe the optimization of a poly(Phe) synthesis system, the conditions of which have been applied for efficient translation of heteropolymeric mRNAs. Here we identify two parameters that are essential to obtain translation at efficiency and accuracy levels equivalent to those in vivo, viz., the fine-tuning of the energy-rich components with an acetyl–phosphate substrate for energy regeneration, as well as the ionic conditions. Applying this system revealed a number of new features: (i) 70S ribosomes are able to recycle within 300 s in a non-enzymatic fashion in the absence of tmRNA. This observation might explain the fact that a knockout of the tmRNA gene ssrA is not lethal for Escherichia coli cells in contrast to other bacterial strains, such as Bacillus subtilis. (ii) The high efficiency of the system was exploited to analyze the misincorporation of various amino acids (resolution limit = 1:15,000). No misreading was observed at the middle codon position and only marginal effects were observed at the first one (even when misreading was artificially stimulated 20- to 30-fold), yielding an improved definition of the near-cognate and non-cognate aminoacyl-tRNAs. (iii) Aminoglycosides increase Phe and Lys incorporation about 2-fold in the presence of poly(U) or poly(UUC) and poly(A), respectively, and induce a back-translocation (except hygromycin B) exclusively in the absence of EF-G•GTP, as do the non-related drugs viomycin and edeine.

Details

einblenden:
ausblenden:
Sprache(n): eng - English
 Datum: 2008-06-27
 Publikationsstatus: Erschienen
 Seiten: -
 Ort, Verlag, Ausgabe: -
 Inhaltsverzeichnis: -
 Art der Begutachtung: -
 Art des Abschluß: -

Veranstaltung

einblenden:

Entscheidung

einblenden:

Projektinformation

einblenden:

Quelle 1

einblenden:
ausblenden:
Titel: Journal of Molecular Biology
  Alternativer Titel : J Mol Biol
Genre der Quelle: Zeitschrift
 Urheber:
Affiliations:
Ort, Verlag, Ausgabe: -
Seiten: - Band / Heft: 380 (1) Artikelnummer: - Start- / Endseite: 193 - 205 Identifikator: -