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  Characterization of RNA-protein interactions by phosphorothioate footprinting and its applications to the ribosome

Bishop, A. Ö. T., Stelzl, U., Pech, M., & Nierhaus, K. H. (2008). Characterization of RNA-protein interactions by phosphorothioate footprinting and its applications to the ribosome. In R.-J. Lin (Ed.), RNA-Protein Interaction Protocols (pp. 129-151). Berlin [et al]: Springer.

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10.1007_978-1-60327-475-3_BookFrontMatter_1.pdf (Any fulltext), 182KB
 
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 Creators:
Bishop, A. Özlem Tastan, Author
Stelzl, Ulrich1, Author           
Pech, Markus2, Author           
Nierhaus, Knud H.3, Author           
Affiliations:
1Molecular Interaction Networks (Ulrich Stelzl), Independent Junior Research Groups (OWL), Max Planck Institute for Molecular Genetics, Max Planck Society, ou_1479660              
2Dept. of Computational Molecular Biology (Head: Martin Vingron), Max Planck Institute for Molecular Genetics, Max Planck Society, ou_1433547              
3Ribosomes, Max Planck Institute for Molecular Genetics, Max Planck Society, ou_1433558              

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Free keywords: Phosphorothioate technique - ribosomal tRNA contact sites - RNA-protein interactions
 Abstract: Analogs of naturally occurring substances obtained by chemical modifications are powerful tools to study intra- and intermolecular interactions. We have used the phosphorothioate technique to analyze RNA-protein interactions, here the interactions of transfer RNAs (tRNAs) with the three ribosomal binding sites. We describe preparation and purification of thioated tRNAs, formation of functional complexes of programmed ribosomes with tRNAs, and the evaluation of the observed phosphorothioate footprints on the tRNAs.

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Language(s): eng - English
 Dates: 2008-10-01
 Publication Status: Issued
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Title: RNA-Protein Interaction Protocols
Source Genre: Book
 Creator(s):
Lin, Ren-Jang, Editor
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Publ. Info: Berlin [et al] : Springer
Pages: - Volume / Issue: - Sequence Number: - Start / End Page: 129 - 151 Identifier: ISBN: 978-1-58829-419-7

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Title: Methos of molecular biology
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