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  Identification of novel transcriptional regulators involved in macrophage differentiation and activation in U937 cells.

Baek, Y.-S., Haas, S., Hackstein, H., Bein, G., Hernandez-Santana, M., Lehrach, H., et al. (2009). Identification of novel transcriptional regulators involved in macrophage differentiation and activation in U937 cells. BMC Immunology, 10, 18-18. doi:10.1186/1471-2172-10-18.

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Genre: Journal Article
Alternative Title : BMC Immunology

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1471-2172-10-18.pdf (Any fulltext), 356KB
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 Creators:
Baek, Young-Sook1, Author           
Haas, Stefan2, Author           
Hackstein, Holger, Author
Bein, Gregor, Author
Hernandez-Santana, Maria, Author
Lehrach, Hans1, Author           
Sauer, Sascha3, Author           
Seitz, Harald1, Author           
Affiliations:
1Dept. of Vertebrate Genomics (Head: Hans Lehrach), Max Planck Institute for Molecular Genetics, Max Planck Society, ou_1433550              
2Gene Structure and Array Design (Stefan Haas), Dept. of Computational Molecular Biology (Head: Martin Vingron), Max Planck Institute for Molecular Genetics, Max Planck Society, ou_1479640              
3Nutrigenomics and Gene Regulation (Sascha Sauer), Independent Junior Research Groups (OWL), Max Planck Institute for Molecular Genetics, Max Planck Society, ou_1479662              

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 Abstract: Background Monocytes and macrophages play essential role in innate immunity. Understanding the underlying mechanism of macrophage differentiation and the identification of regulatory mechanisms will help to find new strategies to prevent their harmful effects in chronic inflammatory diseases and sepsis. Results Maturation of blood monocytes into tissue macrophages and subsequent inflammatory response was mimicked in U937 cells of human histocytic lymphoma origin. Whole genome array analysis was employed to evaluate gene expression profile to identify underlying transcriptional networks implicated during the processes of differentiation and inflammation. In addition to already known transcription factors (i.e. MAFB, EGR, IRF, BCL6, NFkB, AP1, Nur77), gene expression analysis further revealed novel genes (i.e. MEF2, BRI, HLX, HDAC5, H2AV, TCF7L2, NFIL3) previously uncharacterized to be involved in the differentiation process. A total of 58 selected genes representing cytokines, chemokines, surface antigens, signaling molecules and transcription factors were validated by real time PCR and compared to primary monocyte-derived macrophages. Beside the verification of several new genes, the comparison reveals individual heterogeneity of blood donors. Conclusion Up regulation of MEF2 family, HDACs, and H2AV during cell differentiation and inflammation sheds new lights onto regulation events on transcriptional and epigenetic level controlling these processes. Data generated will serve as a source for further investigation of macrophages differentiation pathways and related biological responses.

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Language(s): eng - English
 Dates: 2009-04-02
 Publication Status: Issued
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Title: BMC Immunology
  Alternative Title : BMC Immunology
Source Genre: Journal
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Pages: - Volume / Issue: 10 Sequence Number: - Start / End Page: 18 - 18 Identifier: ISSN: 1471-2172)