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  Single centrosome manipulation reveals its electric charge and associated dynamic structure.

Hormeño, S., Ibarra, B., Chichón, F. J., K. Habermann, K., B.M.H. Lange, B. M. H., Valpuesta, J. M., et al. (2009). Single centrosome manipulation reveals its electric charge and associated dynamic structure. Biophysical Journal, 97(4), 1022-1030. Retrieved from http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B94RW-4X0HHNR-D&_user=28761&_coverDate=08%2F19%2F2009&_rdoc=1&_fmt=high&_orig=search&_sort=d&_docanchor=&view=c&_acct=C000002718&_version=1&_urlVersion=0&_userid=28761&md5=ac0cf97c45b096d7456fc6fa81038b3d.

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Datensatz-Permalink: https://hdl.handle.net/11858/00-001M-0000-0010-7D36-E Versions-Permalink: https://hdl.handle.net/11858/00-001M-0000-0010-7D37-C
Genre: Zeitschriftenartikel
Alternativer Titel : Biophys J.

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1022.pdf (beliebiger Volltext), 768KB
 
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Eingeschränkt (Max Planck Institute for Molecular Genetics, MBMG; )
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 Urheber:
Hormeño, S., Autor
Ibarra, B., Autor
Chichón, F. J., Autor
K. Habermann, K.1, Autor
B.M.H. Lange, B. M. H.1, Autor
Valpuesta, J. M., Autor
Carrascosa, J. L., Autor
Arias-Gonzalez, J. R., Autor
Affiliations:
1Max Planck Society, ou_persistent13              

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Schlagwörter: -
 Zusammenfassung: The centrosome is the major microtubule-organizing center in animal cells and consists of a pair of centrioles surrounded by a pericentriolar material. We demonstrate laser manipulation of individual early Drosophila embryo centrosomes in between two microelectrodes to reveal that it is a net negatively charged organelle with a very low isoelectric region (3.1 ± 0.1). From this single-organelle electrophoresis, we infer an effective charge smaller than or on the order of 103 electrons, which corresponds to a surface-charge density significantly smaller than that of microtubules. We show, however, that the charge of the centrosome has a remarkable influence over its own structure. Specifically, we investigate the hydrodynamic behavior of the centrosome by measuring its size by both Stokes law and thermal-fluctuation spectral analysis of force. We find, on the one hand, that the hydrodynamic size of the centrosome is 60% larger than its electron microscopy diameter, and on the other hand, that this physiological expansion is produced by the electric field that drains to the centrosome, a self-effect that modulates its structural behavior via environmental pH. This methodology further proves useful for studying the action of different environmental conditions, such as the presence of Ca2+, over the thermally induced dynamic structure of the centrosome.

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Sprache(n): eng - English
 Datum: 2009-08-19
 Publikationsstatus: Erschienen
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 Identifikatoren: eDoc: 456966
URI: http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B94RW-4X0HHNR-D&_user=28761&_coverDate=08%2F19%2F2009&_rdoc=1&_fmt=high&_orig=search&_sort=d&_docanchor=&view=c&_acct=C000002718&_version=1&_urlVersion=0&_userid=28761&md5=ac0cf97c45b096d7456fc6fa81038b3d
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Titel: Biophysical Journal
  Alternativer Titel : Biophys J.
Genre der Quelle: Zeitschrift
 Urheber:
Affiliations:
Ort, Verlag, Ausgabe: -
Seiten: - Band / Heft: 97 (4) Artikelnummer: - Start- / Endseite: 1022 - 1030 Identifikator: ISSN: 1542-0086