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  Large-scale sorting of C. elegans embryos reveals the dynamics of small RNA expression

Stoeckius, M., Maaskola, J., Colombo, T., Rahn, H.-P., Friedländer, M. R., Li, N., et al. (2009). Large-scale sorting of C. elegans embryos reveals the dynamics of small RNA expression. Nature Methods, 6, 745-751. doi:10.1038/nmeth.1370.

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Genre: Journal Article
Alternative Title : Nat Mathods

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Stoeckius, Marlon, Author
Maaskola, Jonas, Author
Colombo, Teresa, Author
Rahn, Hans-Peter, Author
Friedländer, Marc R., Author
Li, Na1, Author           
Chen, Wei2, Author           
Piano, Fabio, Author
Rajewsky, Nikolaus, Author
Affiliations:
1Computational Epigenetics (Ho-Ryun Chung), Independent Junior Research Groups (OWL), Max Planck Institute for Molecular Genetics, Max Planck Society, ou_1479658              
2Dept. of Human Molecular Genetics (Head: Hans-Hilger Ropers), Max Planck Institute for Molecular Genetics, Max Planck Society, ou_1433549              

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 Abstract: Caenorhabditis elegans is one of the most prominent model systems for embryogenesis, but collecting many precisely staged embryos has been impractical. Thus, early C. elegans embryogenesis has not been amenable to most high-throughput genomics or biochemistry assays. To overcome this problem, we devised a method to collect staged C. elegans embryos by fluorescence-activated cell sorting (eFACS). In a proof-of-principle experiment, we found that a single eFACS run routinely yielded tens of thousands of almost perfectly staged 1-cell stage embryos. As the earliest embryonic events are driven by posttranscriptional regulation, we combined eFACS with second-generation sequencing to profile the embryonic expression of small, noncoding RNAs. We discovered complex and orchestrated changes in the expression between and within almost all classes of small RNAs, including microRNAs and 26G-RNAs, during embryogenesis.

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Language(s): eng - English
 Dates: 2009-09-06
 Publication Status: Issued
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Title: Nature Methods
  Alternative Title : Nat Mathods
Source Genre: Journal
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Pages: - Volume / Issue: 6 Sequence Number: - Start / End Page: 745 - 751 Identifier: ISSN: 1548-7091