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  Quantification of target proteins using hydrogel antibody arrays and MALDI time-of-flight mass spectrometry (A2M2S)

Darii, E., Lebeau, D., Papin, N., Rubina, A. Y., Stomakhin, A., Tost, J., et al. (2009). Quantification of target proteins using hydrogel antibody arrays and MALDI time-of-flight mass spectrometry (A2M2S). New Biotechnology, 25(6), 404-416. doi:10.1016/j.nbt.2009.03.001.

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Darii, Ekaterina, Autor
Lebeau, Diane, Autor
Papin, Nelly, Autor
Rubina, Alla Y., Autor
Stomakhin, Andrei, Autor
Tost, Jörg, Autor
Sauer, Sascha1, Autor           
Savvateeva, Elena, Autor
Dementieva, Ekaterina, Autor
Zasedatelev, Alexander, Autor
Makarov, Alexander A., Autor
Gut, Ivo G., Autor
Affiliations:
1Nutrigenomics and Gene Regulation (Sascha Sauer), Independent Junior Research Groups (OWL), Max Planck Institute for Molecular Genetics, Max Planck Society, ou_1479662              

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 Zusammenfassung: Mass spectrometry-based analysis techniques are widely applied in proteomics. This study presents a novel method for quantitative multiplex candidate protein profiling. It applies immunocapture of differentially labeled protein complements on hydrogel antibody arrays and subsequent quantification by MS. To make this approach quantitative a labeling approach was devised. The impact of labeling on the antibody/antigen interaction was assessed in detail by surface plasmon resonance. Owing to the resolution by mass more than two protein samples can be compared simultaneously. Direct labeling of crude samples such as sera was developed and so enables the absolute quantification of target proteins straight from crude samples without a protein purification step. It was used to measure the concentration of apolipoprotein A-1 in serum. This method has been termed A2M2S for Affinity Arrays and MALDI Mass Spectrometry.

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Sprache(n): eng - English
 Datum: 2009-09-15
 Publikationsstatus: Erschienen
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Titel: New Biotechnology
Genre der Quelle: Zeitschrift
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Ort, Verlag, Ausgabe: -
Seiten: - Band / Heft: 25 (6) Artikelnummer: - Start- / Endseite: 404 - 416 Identifikator: ISSN: 1871-6784